Clone: 2840487120807852 A05

Tagged gene: ligatin
Identifier: FBgn0041588

Clone FlyFos024773(pRedFlp-Hgr)(ligatin[30149]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
24983847 24990694 15120 21967 - gene CG11897 coding_transcript
24979185 24982981 22833 26629 - gene Rpn2 coding_transcript
24977793 24978969 26845 28021 + gene Slbp coding_transcript
24975618 24977512 28302 31258 + gene ligatin coding_transcript
24975618 24977512 28302 31258 + mrna FBtr0085410 coding_transcript
24977012 24977512 28302 28802 + exon coding_transcript
24977412 24977512 28302 28402 + five_prime_utr coding_transcript
24977012 24977411 28403 28802 + cds coding_transcript
24976954 24977011 28803 28860 + intron coding_transcript
24975618 24976953 28861 31258 + exon coding_transcript
24975662 24976953 28861 31214 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 30150 30209 + cds 2xTY1 CLC
1548 2264 30216 30932 + cds SGFP CLC
2271 2312 30939 30980 + cds V5 CLC
2313 2336 30981 31004 + cds Precision cut site CLC
2337 2357 31005 31025 + cds TEV CLC
2358 2429 31026 31097 + cds BLRP CLC
3813 3846 31105 31138 + misc_recomb FRT CLC
dmel-5.43-3R
24975618 24975661 31215 31258 + three_prime_utr coding_transcript
24973970 24975623 31253 32906 - gene Pglym78 coding_transcript
24972738 24973894 32982 34138 + gene CG11882 coding_transcript
24971400 24972615 34261 35476 + gene CG14511 coding_transcript

Sets

Oligos

Name/Sequence Export
ligatin[30149]::S000169_fly_pretag_fwd
GCATCCTGGGCCTGGAATTGGCCAAGGACGGCAAGAAGAATAAGAAGAAGgaagtgcataccaatcaggacccgc
fasta Fasta
ligatin[30149]::S000169_fly_pretag_rev
ATTACACTCAAGAGGACAGGCGGAGGAGGCTTCATTTATTGTCCGGCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta