Clone: 2840487120807852 G04

Tagged gene: Sfp84E
Identifier: FBgn0259974

Clone FlyFos024768(pRedFlp-Hgr)(Sfp84E[28763]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
3807539 3821568 12681 26710 + gene CG2781 coding_transcript
3805310 3806044 28205 30001 + gene Sfp84E coding_transcript
3805310 3806044 28205 30001 + mrna FBtr0300322 coding_transcript
3806013 3806044 28205 28236 + exon coding_transcript
3806016 3806044 28205 28233 + five_prime_utr coding_transcript
3806013 3806015 28234 28236 + cds coding_transcript
3805963 3806012 28237 28286 + intron coding_transcript
3805310 3805962 28287 30001 + exon coding_transcript
3805483 3805962 28287 29828 + cds coding_transcript
3804681 3805546 28703 30630 - gene Os-C coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 28764 28823 + cds 2xTY1 CLC
1548 2264 28830 29546 + cds SGFP CLC
2271 2312 29553 29594 + cds V5 CLC
2313 2336 29595 29618 + cds Precision cut site CLC
2337 2357 29619 29639 + cds TEV CLC
2358 2429 29640 29711 + cds BLRP CLC
3813 3846 29719 29752 + misc_recomb FRT CLC
dmel-5.43-3R
3805310 3805482 29829 30001 + three_prime_utr coding_transcript
3800128 3803488 31823 35183 + gene CD98hc coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Sfp84E[28763]::S000169_fly_pretag_rev
ATTATTTTATTTTGGTTACATCAATATATAAGCTCTCAGATTGTTGTTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Sfp84E[28763]::S000169_fly_pretag_fwd
GCTATTATATTTTAACCAGTGCGGAAAACGAAATAGTTGAATCAAAAGGTgaagtgcataccaatcaggacccgc
fasta Fasta