Clone: 35649249831002394 B04

Tagged gene: Jhe
Identifier: FBgn0010052

Clone FlyFos024388(pRedFlp-Hgr)(Jhe[25162]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
11994945 11997520 9963 12538 - gene Ric coding_transcript
11997990 12004501 13008 19519 + gene Asph coding_transcript
12004614 12006654 19632 21672 + gene Jhedup coding_transcript
12007311 12010278 22329 26358 + gene Jhe coding_transcript
12007311 12010278 22329 26358 + mrna FBtr0087223 coding_transcript
12007311 12007419 22329 22437 + exon coding_transcript
12007311 12007419 22329 22437 + five_prime_utr coding_transcript
12007420 12007903 22438 22921 + intron coding_transcript
12007656 12010278 22674 26358 + mrna FBtr0301751 coding_transcript
12007656 12007713 22674 22731 + exon coding_transcript
12007656 12007713 22674 22731 + five_prime_utr coding_transcript
12007714 12007903 22732 22921 + intron coding_transcript
12007904 12008158 22922 23176 + exon coding_transcript
12007904 12008158 22922 23176 + exon coding_transcript
12007904 12007936 22922 22954 + five_prime_utr coding_transcript
12007904 12007936 22922 22954 + five_prime_utr coding_transcript
12007937 12008158 22955 23176 + cds coding_transcript
12007937 12008158 22955 23176 + cds coding_transcript
12008159 12008220 23177 23238 + intron coding_transcript
12008159 12008220 23177 23238 + intron coding_transcript
12008221 12008364 23239 23382 + exon coding_transcript
12008221 12008364 23239 23382 + cds coding_transcript
12008221 12008364 23239 23382 + exon coding_transcript
12008221 12008364 23239 23382 + cds coding_transcript
12008365 12008470 23383 23488 + intron coding_transcript
12008365 12008470 23383 23488 + intron coding_transcript
12008471 12008621 23489 23639 + exon coding_transcript
12008471 12008621 23489 23639 + cds coding_transcript
12008471 12008621 23489 23639 + exon coding_transcript
12008471 12008621 23489 23639 + cds coding_transcript
12008622 12008687 23640 23705 + intron coding_transcript
12008622 12008687 23640 23705 + intron coding_transcript
12008688 12009430 23706 24448 + exon coding_transcript
12008688 12009430 23706 24448 + cds coding_transcript
12008688 12009430 23706 24448 + exon coding_transcript
12008688 12009430 23706 24448 + cds coding_transcript
12009431 12009601 24449 24619 + intron coding_transcript
12009431 12009601 24449 24619 + intron coding_transcript
12009602 12009891 24620 24909 + exon coding_transcript
12009602 12009891 24620 24909 + cds coding_transcript
12009602 12009891 24620 24909 + exon coding_transcript
12009602 12009891 24620 24909 + cds coding_transcript
12009892 12009957 24910 24975 + intron coding_transcript
12009892 12009957 24910 24975 + intron coding_transcript
12009958 12010278 24976 26358 + exon coding_transcript
12009958 12010278 24976 26358 + exon coding_transcript
12009958 12010147 24976 26227 + cds coding_transcript
12009958 12010147 24976 26227 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 25163 25222 + cds 2xTY1 CLC
1548 2264 25229 25945 + cds SGFP CLC
2271 2312 25952 25993 + cds V5 CLC
2313 2336 25994 26017 + cds Precision cut site CLC
2337 2357 26018 26038 + cds TEV CLC
2358 2429 26039 26110 + cds BLRP CLC
3813 3846 26118 26151 + misc_recomb FRT CLC
dmel-5.43-2R
12010148 12010278 26228 26358 + three_prime_utr coding_transcript
12010148 12010278 26228 26358 + three_prime_utr coding_transcript
12010989 12012082 27069 28162 + gene CG30095 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Jhe[25162]::S000169_fly_pretag_fwd
AATTCCAAACGGATAGGGTGAATCTATGGTCGCATATACTCAACGAAAAGgaagtgcataccaatcaggacccgc
 fasta Fasta
Jhe[25162]::S000169_fly_pretag_rev
TCATATATGTACATTAGTTTATCGGTTGAGCAGAGTTTAGAACTATTTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta