Clone: 6005613252476711 C12

Tagged gene: bif
Identifier: FBgn0014133

Clone FlyFos024310(pRedFlp-Hgr)(bif[31691]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
11571376 11572584 15099 16307 - gene CG2371 coding_transcript
11573028 11588774 16751 33559 + gene bif coding_transcript
11573028 11588774 16751 33559 + mrna FBtr0073527 coding_transcript
11573028 11588766 16751 33551 + mrna FBtr0073526 coding_transcript
11573028 11573322 16751 17045 + exon coding_transcript
11573028 11573322 16751 17045 + five_prime_utr coding_transcript
11573028 11573322 16751 17045 + exon coding_transcript
11573028 11573322 16751 17045 + five_prime_utr coding_transcript
11573323 11574876 17046 18599 + intron coding_transcript
11573323 11574876 17046 18599 + intron coding_transcript
11574877 11575147 18600 18870 + exon coding_transcript
11574877 11575147 18600 18870 + exon coding_transcript
11574877 11574885 18600 18608 + five_prime_utr coding_transcript
11574877 11574885 18600 18608 + five_prime_utr coding_transcript
11574886 11575147 18609 18870 + cds coding_transcript
11574886 11575147 18609 18870 + cds coding_transcript
11575148 11575750 18871 19473 + intron coding_transcript
11575148 11575750 18871 19473 + intron coding_transcript
11575751 11578287 19474 22010 + exon coding_transcript
11575751 11578287 19474 22010 + cds coding_transcript
11575751 11578287 19474 22010 + exon coding_transcript
11575751 11578287 19474 22010 + cds coding_transcript
11578288 11581494 22011 25217 + intron coding_transcript
11578288 11581494 22011 25217 + intron coding_transcript
11581495 11581668 25218 25391 + exon coding_transcript
11581495 11581668 25218 25391 + cds coding_transcript
11581495 11581668 25218 25391 + exon coding_transcript
11581495 11581668 25218 25391 + cds coding_transcript
11581669 11583233 25392 26956 + intron coding_transcript
11581669 11583233 25392 26956 + intron coding_transcript
11583234 11583576 26957 27299 + exon coding_transcript
11583234 11583576 26957 27299 + cds coding_transcript
11583234 11583576 26957 27299 + exon coding_transcript
11583234 11583576 26957 27299 + cds coding_transcript
11583577 11587579 27300 31302 + intron coding_transcript
11583577 11586831 27300 30554 + intron coding_transcript
11586832 11587210 30555 30933 + exon coding_transcript
11586832 11586842 30555 30565 + cds coding_transcript
11586843 11587210 30566 30933 + three_prime_utr coding_transcript
11587211 11587579 30934 31302 + intron coding_transcript
11587580 11588774 31303 33559 + exon coding_transcript
11587580 11588774 31303 33559 + three_prime_utr coding_transcript
11587580 11588766 31303 33551 + exon coding_transcript
11587580 11587971 31303 32756 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 31692 31751 + cds 2xTY1 CLC
1548 2264 31758 32474 + cds SGFP CLC
2271 2312 32481 32522 + cds V5 CLC
2313 2336 32523 32546 + cds Precision cut site CLC
2337 2357 32547 32567 + cds TEV CLC
2358 2429 32568 32639 + cds BLRP CLC
3813 3846 32647 32680 + misc_recomb FRT CLC
dmel-5.43-X
11587972 11588766 32757 33551 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
bif[31691]::S000169_fly_pretag_rev
GGTAGAAAAAGAGCTAGACGCTCCGGTTTTTTGGCTGGCAGGACGACCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
bif[31691]::S000169_fly_pretag_fwd
GCGCCGAAGTTGTGTACAGCGAGGGTACACAGAAAACGGATTTGCTATATgaagtgcataccaatcaggacccgc
 fasta Fasta