Clone: 6005613252476711 H10

Tagged gene: Mat1
Identifier: FBgn0024956

Clone FlyFos024219(pRedFlp-Hgr)(Mat1[30005]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
6626122 6627339 28932 31211 + gene Mat1 coding_transcript
6626122 6627339 28932 31211 + mrna FBtr0088255 coding_transcript
6626122 6627339 28932 31211 + exon coding_transcript
6627226 6627339 28932 29045 + five_prime_utr coding_transcript
6626263 6627225 29046 31070 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 30006 30065 + cds 2xTY1 CLC
1548 2264 30072 30788 + cds SGFP CLC
2271 2312 30795 30836 + cds V5 CLC
2313 2336 30837 30860 + cds Precision cut site CLC
2337 2357 30861 30881 + cds TEV CLC
2358 2429 30882 30953 + cds BLRP CLC
3813 3846 30961 30994 + misc_recomb FRT CLC
dmel-5.43-2R
6626122 6626262 31071 31211 + three_prime_utr coding_transcript
6618861 6626116 31217 38472 - gene CG7220 coding_transcript
6617080 6618419 38914 40253 + gene CG12338 coding_transcript
6608833 6616695 40638 48500 + gene Rab3 coding_transcript
6612775 6614783 42550 44558 - gene CG12309 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Mat1[30005]::S000169_fly_pretag_rev
TTCAGTGCTCTGGTTGTGCGACCATTAAAGGATATTTTTTTAGAGAGCTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Mat1[30005]::S000169_fly_pretag_fwd
CGCTGCAGGGACTCTACTATACGGCTACAACGGGTGTGGTATCTGGCACCgaagtgcataccaatcaggacccgc
fasta Fasta