Clone: 13820775239620986 B10

Tagged gene: Sgs5
Identifier: FBgn0003375

Clone FlyFos024067(pRedFlp-Hgr)(Sgs5[31085]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
13420108 13420783 29476 30151 + gene CG7587 coding_transcript
13421084 13421834 30452 32264 + gene Sgs5 coding_transcript
13421084 13421834 30452 32264 + mrna FBtr0083461 coding_transcript
13421084 13421381 30452 30749 + exon coding_transcript
13421084 13421113 30452 30481 + five_prime_utr coding_transcript
13421114 13421381 30482 30749 + cds coding_transcript
13421382 13421437 30750 30805 + intron coding_transcript
13421438 13421626 30806 30994 + exon coding_transcript
13421438 13421626 30806 30994 + cds coding_transcript
13421627 13421685 30995 31053 + intron coding_transcript
13421686 13421834 31054 32264 + exon coding_transcript
13421686 13421720 31054 32150 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 31086 31145 + cds 2xTY1 CLC
1548 2264 31152 31868 + cds SGFP CLC
2271 2312 31875 31916 + cds V5 CLC
2313 2336 31917 31940 + cds Precision cut site CLC
2337 2357 31941 31961 + cds TEV CLC
2358 2429 31962 32033 + cds BLRP CLC
3813 3846 32041 32074 + misc_recomb FRT CLC
dmel-5.43-3R
13421721 13421834 32151 32264 + three_prime_utr coding_transcript
13422258 13422639 32688 33069 + gene CG7606 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Sgs5[31085]::S000169_fly_pretag_fwd
CATTTTGTATCTTTACAGCATTCCTGACTTATTCCTATTGTTGGCCCTTCgaagtgcataccaatcaggacccgc
fasta Fasta
Sgs5[31085]::S000169_fly_pretag_rev
AAACAAACAAAGATATTAGTAACGTGATCCAACTGAATCCACCTCACTTActtgtcgtcgtcatccttgtagtca
fasta Fasta