Clone: 13820775239620986 F09

Tagged gene: pie
Identifier: FBgn0005683

Clone FlyFos024058(pRedFlp-Hgr)(pie[35861]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
10439440 10440886 15806 17252 - gene CG13142 coding_transcript
10437551 10439244 17448 19141 - gene CHIP coding_transcript
10435991 10437317 19375 20701 - gene CG5198 coding_transcript
10432677 10435679 21013 24015 + gene CG5337 coding_transcript
10431344 10432999 23693 25348 - gene TfIIB coding_transcript
10429920 10430980 25712 26772 + gene CG5343 coding_transcript
10428833 10429988 26704 27859 - gene CG5188 coding_transcript
10426867 10428632 28060 29825 - gene KdelR coding_transcript
10425483 10426791 29901 31209 + gene SmB coding_transcript
10423291 10425482 31210 33401 - gene CG5168 coding_transcript
10420711 10422781 33911 37043 + gene pie coding_transcript
10420711 10422781 33911 37043 + mrna FBtr0080046 coding_transcript
10422530 10422781 33911 34162 + exon coding_transcript
10422644 10422781 33911 34048 + five_prime_utr coding_transcript
10422530 10422643 34049 34162 + cds coding_transcript
10422463 10422529 34163 34229 + intron coding_transcript
10420711 10422462 34230 37043 + exon coding_transcript
10420828 10422462 34230 36926 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 35862 35921 + cds 2xTY1 CLC
1548 2264 35928 36644 + cds SGFP CLC
2271 2312 36651 36692 + cds V5 CLC
2313 2336 36693 36716 + cds Precision cut site CLC
2337 2357 36717 36737 + cds TEV CLC
2358 2429 36738 36809 + cds BLRP CLC
3813 3846 36817 36850 + misc_recomb FRT CLC
dmel-5.43-2L
10420711 10420827 36927 37043 + three_prime_utr coding_transcript
10419190 10420612 37142 38564 - gene RfC3 coding_transcript
10414034 10418991 38763 43720 - gene Klp31E coding_transcript
10410537 10413572 44182 47217 + gene CG5355 coding_transcript
10408351 10410467 47287 49403 - gene RpS27A coding_transcript
10408353 10409604 48150 49401 - gene Ip259 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
pie[35861]::S000169_fly_pretag_fwd
TTGAGCATATCGAGAAGTTCCACGAATGGTACAGATCAGAGGGGTTCTCTgaagtgcataccaatcaggacccgc
fasta Fasta
pie[35861]::S000169_fly_pretag_rev
GAATTGTTTAAAACATTAAGAACATTAAGAATTGTTTTAAACAATATTTActtgtcgtcgtcatccttgtagtca
fasta Fasta