Clone: 13820775239620986 A08

Tagged gene: TwdlT
Identifier: FBgn0029170

Clone FlyFos024032(pRedFlp-Hgr)(TwdlT[23803]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
22883617 22889500 10471 16354 + gene Nep5 coding_transcript
22887938 22888783 11188 12033 - gene CG34292 coding_transcript
22853640 22883661 16310 47393 - gene NepYr coding_transcript
22874947 22876742 23229 26086 - gene TwdlT coding_transcript
22874947 22876742 23229 26086 - mrna FBtr0085101 coding_transcript
22875644 22876742 23229 25389 - exon coding_transcript
22876171 22876742 23229 23800 - three_prime_utr coding_transcript
22875644 22876170 23801 25389 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 23877 23910 - misc_recomb FRT CLC
2358 2429 23918 23989 - cds BLRP CLC
2337 2357 23990 24010 - cds TEV CLC
2313 2336 24011 24034 - cds Precision cut site CLC
2271 2312 24035 24076 - cds V5 CLC
1548 2264 24083 24799 - cds SGFP CLC
1482 1541 24806 24865 - cds 2xTY1 CLC
dmel-5.43-3R
22875568 22875643 25390 25465 - intron coding_transcript
22875113 22875567 25466 25920 - exon coding_transcript
22875234 22875567 25466 25799 - cds coding_transcript
22875113 22875233 25800 25920 - five_prime_utr coding_transcript
22875011 22875112 25921 26022 - intron coding_transcript
22874947 22875010 26023 26086 - exon coding_transcript
22874947 22875010 26023 26086 - five_prime_utr coding_transcript
22850683 22851822 49211 50350 + gene CG6271 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
TwdlT[23803]::S000169_fly_pretag_rev
GAGTTCGAAGTTCGCAATTTTGAGTTCACGATGGGTTGAAGATCCATTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
TwdlT[23803]::S000169_fly_pretag_fwd
TCTCGGCCCCGTCCAGCAACTACGGACCACCTGGAAAGTCCGGACCCTACgaagtgcataccaatcaggacccgc
fasta Fasta