Clone: 13820775239620986 G07

Tagged gene: CG31477
Identifier: FBgn0051477

Clone FlyFos024027(pRedFlp-Hgr)(CG31477[23958]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
5948087 5951383 20515 23811 + gene knk coding_transcript
5947523 5948042 23856 25437 - gene CG31477 coding_transcript
5947523 5948042 23856 25437 - mrna FBtr0113408 coding_transcript
5947727 5948042 23856 25233 - exon coding_transcript
5947943 5948042 23856 23955 - three_prime_utr coding_transcript
5947523 5948034 23864 25437 - mrna FBtr0082180 coding_transcript
5947727 5948034 23864 25233 - exon coding_transcript
5947943 5948034 23864 23955 - three_prime_utr coding_transcript
5947748 5947942 23956 25212 - cds coding_transcript
5947748 5947942 23956 25212 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 24032 24065 - misc_recomb FRT CLC
2358 2429 24073 24144 - cds BLRP CLC
2337 2357 24145 24165 - cds TEV CLC
2313 2336 24166 24189 - cds Precision cut site CLC
2271 2312 24190 24231 - cds V5 CLC
1548 2264 24238 24954 - cds SGFP CLC
1482 1541 24961 25020 - cds 2xTY1 CLC
dmel-5.43-3R
5947727 5947747 25213 25233 - five_prime_utr coding_transcript
5947727 5947747 25213 25233 - five_prime_utr coding_transcript
5947669 5947726 25234 25291 - intron coding_transcript
5947669 5947726 25234 25291 - intron coding_transcript
5947523 5947668 25292 25437 - exon coding_transcript
5947523 5947668 25292 25437 - five_prime_utr coding_transcript
5947523 5947668 25292 25437 - exon coding_transcript
5947523 5947668 25292 25437 - five_prime_utr coding_transcript
5946121 5947122 25838 26839 + gene CG6208 coding_transcript
5938268 5945142 27818 34692 - gene CG3940 coding_transcript
5927142 5935843 37117 45818 + gene Fmr1 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG31477[23958]::S000169_fly_pretag_rev
CTGTTATTCTGGATGAAACATGGTGTACAAATCACCGAAATGGGCGCCTActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG31477[23958]::S000169_fly_pretag_fwd
TGAACGGAAAGCCTGTGCCGCGCAAGAAGGTAGAACGTGAATCCGAATCTgaagtgcataccaatcaggacccgc
fasta Fasta