Clone: 13820775239620986 G04

Tagged gene: Top1
Identifier: FBgn0004924

Clone FlyFos023991(pRedFlp-Hgr)(Top1[23251]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
15213525 15222062 16828 26427 + gene Top1 coding_transcript
15213525 15222062 16828 26427 + mrna FBtr0074005 coding_transcript
15213525 15222062 16828 26427 + mrna FBtr0100294 coding_transcript
15213525 15213840 16828 17143 + exon coding_transcript
15213525 15213840 16828 17143 + exon coding_transcript
15213525 15213813 16828 17116 + five_prime_utr coding_transcript
15213525 15213813 16828 17116 + five_prime_utr coding_transcript
15213814 15213840 17117 17143 + cds coding_transcript
15213814 15213840 17117 17143 + cds coding_transcript
15213841 15215186 17144 18489 + intron coding_transcript
15213841 15215186 17144 18489 + intron coding_transcript
15214402 15220992 17705 25357 + mrna FBtr0308699 coding_transcript
15214402 15215065 17705 18368 + exon coding_transcript
15214402 15215050 17705 18353 + five_prime_utr coding_transcript
15215051 15215065 18354 18368 + cds coding_transcript
15215066 15215186 18369 18489 + intron coding_transcript
15215187 15215404 18490 18707 + exon coding_transcript
15215187 15215404 18490 18707 + cds coding_transcript
15215187 15215404 18490 18707 + exon coding_transcript
15215187 15215404 18490 18707 + cds coding_transcript
15215187 15215404 18490 18707 + exon coding_transcript
15215187 15215404 18490 18707 + cds coding_transcript
15215405 15215893 18708 19196 + intron coding_transcript
15215405 15215893 18708 19196 + intron coding_transcript
15215405 15215893 18708 19196 + intron coding_transcript
15215894 15216860 19197 20163 + exon coding_transcript
15215894 15216860 19197 20163 + cds coding_transcript
15215894 15216860 19197 20163 + exon coding_transcript
15215894 15216860 19197 20163 + cds coding_transcript
15215894 15216860 19197 20163 + exon coding_transcript
15215894 15216860 19197 20163 + cds coding_transcript
15216861 15216931 20164 20234 + intron coding_transcript
15216861 15216931 20164 20234 + intron coding_transcript
15216861 15216931 20164 20234 + intron coding_transcript
15216879 15220564 20182 24929 + mrna FBtr0074006 coding_transcript
15216879 15217008 20182 20311 + exon coding_transcript
15216879 15216898 20182 20201 + five_prime_utr coding_transcript
15216899 15217008 20202 20311 + cds coding_transcript
15216932 15217008 20235 20311 + exon coding_transcript
15216932 15217008 20235 20311 + cds coding_transcript
15216932 15217008 20235 20311 + exon coding_transcript
15216932 15217008 20235 20311 + cds coding_transcript
15216932 15217008 20235 20311 + exon coding_transcript
15216932 15217008 20235 20311 + cds coding_transcript
15217009 15218103 20312 21406 + intron coding_transcript
15217009 15218103 20312 21406 + intron coding_transcript
15217009 15218103 20312 21406 + intron coding_transcript
15217009 15218103 20312 21406 + intron coding_transcript
15218104 15219233 21407 22536 + exon coding_transcript
15218104 15219233 21407 22536 + cds coding_transcript
15218104 15219233 21407 22536 + exon coding_transcript
15218104 15219233 21407 22536 + cds coding_transcript
15218104 15219233 21407 22536 + exon coding_transcript
15218104 15219233 21407 22536 + cds coding_transcript
15218104 15219233 21407 22536 + exon coding_transcript
15218104 15219233 21407 22536 + cds coding_transcript
15219234 15219307 22537 22610 + intron coding_transcript
15219234 15219307 22537 22610 + intron coding_transcript
15219234 15219307 22537 22610 + intron coding_transcript
15219234 15219307 22537 22610 + intron coding_transcript
15219308 15219558 22611 22861 + exon coding_transcript
15219308 15219558 22611 22861 + cds coding_transcript
15219308 15219558 22611 22861 + exon coding_transcript
15219308 15219558 22611 22861 + cds coding_transcript
15219308 15219558 22611 22861 + exon coding_transcript
15219308 15219558 22611 22861 + cds coding_transcript
15219308 15219558 22611 22861 + exon coding_transcript
15219308 15219558 22611 22861 + cds coding_transcript
15219559 15219619 22862 22922 + intron coding_transcript
15219559 15219619 22862 22922 + intron coding_transcript
15219559 15219619 22862 22922 + intron coding_transcript
15219559 15219619 22862 22922 + intron coding_transcript
15219620 15219771 22923 23074 + exon coding_transcript
15219620 15219771 22923 23074 + cds coding_transcript
15219620 15219771 22923 23074 + exon coding_transcript
15219620 15219771 22923 23074 + cds coding_transcript
15219620 15219771 22923 23074 + exon coding_transcript
15219620 15219771 22923 23074 + cds coding_transcript
15219620 15219771 22923 23074 + exon coding_transcript
15219620 15219771 22923 23074 + cds coding_transcript
15219772 15219848 23075 23151 + intron coding_transcript
15219772 15219848 23075 23151 + intron coding_transcript
15219772 15219848 23075 23151 + intron coding_transcript
15219772 15219848 23075 23151 + intron coding_transcript
15219849 15222062 23152 26427 + exon coding_transcript
15219849 15220992 23152 25357 + exon coding_transcript
15219849 15220564 23152 24929 + exon coding_transcript
15219849 15219992 23152 24357 + exon coding_transcript
15219849 15219951 23152 24316 + cds coding_transcript
15219849 15219951 23152 24316 + cds coding_transcript
15219849 15219951 23152 24316 + cds coding_transcript
15219849 15219951 23152 24316 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 23252 23311 + cds 2xTY1 CLC
1548 2264 23318 24034 + cds SGFP CLC
2271 2312 24041 24082 + cds V5 CLC
2313 2336 24083 24106 + cds Precision cut site CLC
2337 2357 24107 24127 + cds TEV CLC
2358 2429 24128 24199 + cds BLRP CLC
3813 3846 24207 24240 + misc_recomb FRT CLC
dmel-5.43-X
15219952 15222062 24317 26427 + three_prime_utr coding_transcript
15219952 15220992 24317 25357 + three_prime_utr coding_transcript
15219952 15220564 24317 24929 + three_prime_utr coding_transcript
15219952 15219992 24317 24357 + three_prime_utr coding_transcript
15219993 15220377 24358 24742 + intron coding_transcript
15220378 15222062 24743 26427 + exon coding_transcript
15220378 15222062 24743 26427 + three_prime_utr coding_transcript
15222379 15225035 26744 29400 + gene dah coding_transcript
15224817 15227034 29182 31399 - gene CG9114 coding_transcript
15227343 15234393 31708 38758 + gene HDAC6 coding_transcript
15234406 15236144 38771 40509 - gene CG9123 coding_transcript
15236890 15238654 41255 43019 - gene CG12608 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Top1[23251]::S000169_fly_pretag_rev
GCGTACTTGTGTGTTTGTGTCTAGGCGTGGCTGTGAGGAGAACCCATTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Top1[23251]::S000169_fly_pretag_fwd
GAACCAAATTTCTGTGGGCCGTTCATATGGCCGATGAGAATTATCGTTTCgaagtgcataccaatcaggacccgc
fasta Fasta