Clone: 13820775239620986 B04

Tagged gene: Npc2g
Identifier: FBgn0039800

Clone FlyFos023974(pRedFlp-Hgr)(Npc2g[30223]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
26571201 26572010 28692 29501 + gene Npc2h coding_transcript
26570326 26570983 29719 31438 + gene Npc2g coding_transcript
26570326 26570983 29719 31438 + mrna FBtr0085723 coding_transcript
26570326 26570983 29719 31438 + exon coding_transcript
26570394 26570983 29719 31370 + mrna FBtr0306293 coding_transcript
26570394 26570983 29719 31370 + exon coding_transcript
26570956 26570983 29719 29746 + five_prime_utr coding_transcript
26570956 26570983 29719 29746 + five_prime_utr coding_transcript
26570476 26570955 29747 31288 + cds coding_transcript
26570476 26570955 29747 31288 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 30224 30283 + cds 2xTY1 CLC
1548 2264 30290 31006 + cds SGFP CLC
2271 2312 31013 31054 + cds V5 CLC
2313 2336 31055 31078 + cds Precision cut site CLC
2337 2357 31079 31099 + cds TEV CLC
2358 2429 31100 31171 + cds BLRP CLC
3813 3846 31179 31212 + misc_recomb FRT CLC
dmel-5.43-3R
26570326 26570475 31289 31438 + three_prime_utr coding_transcript
26570394 26570475 31289 31370 + three_prime_utr coding_transcript
26568602 26569637 32127 33162 - gene CG15543 coding_transcript
26555559 26567010 34754 46205 + gene CG11313 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Npc2g[30223]::S000169_fly_pretag_rev
GGTATATTTCACTATTTAATTTAGTGCTCATGCAGCGTGGGCACAAATCActtgtcgtcgtcatccttgtagtca
fasta Fasta
Npc2g[30223]::S000169_fly_pretag_fwd
CCGTCTCCCAGAAGCGCTGCTGCTTCACCATCGACATTAAGGTGGTGCGCgaagtgcataccaatcaggacccgc
fasta Fasta