Clone: 13820775239620986 A02

Tagged gene: Hsp60D
Identifier: FBgn0032525

Clone FlyFos023933(pRedFlp-Hgr)(Hsp60D[29679]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
13459773 13460190 17162 17579 - gene CG42810 coding_transcript
13457636 13458073 19279 19716 - gene CG42809 coding_transcript
13447538 13449497 27855 30876 + gene Hsp60D coding_transcript
13447538 13449497 27855 30876 + mrna FBtr0080517 coding_transcript
13449437 13449497 27855 27915 + exon coding_transcript
13449437 13449497 27855 27915 + five_prime_utr coding_transcript
13447538 13449491 27861 30876 + mrna FBtr0080518 coding_transcript
13447538 13449491 27861 30876 + exon coding_transcript
13449347 13449491 27861 28005 + five_prime_utr coding_transcript
13449371 13449436 27916 27981 + intron coding_transcript
13447538 13449370 27982 30876 + exon coding_transcript
13449347 13449370 27982 28005 + five_prime_utr coding_transcript
13447670 13449346 28006 30744 + cds coding_transcript
13447670 13449346 28006 30744 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 29680 29739 + cds 2xTY1 CLC
1548 2264 29746 30462 + cds SGFP CLC
2271 2312 30469 30510 + cds V5 CLC
2313 2336 30511 30534 + cds Precision cut site CLC
2337 2357 30535 30555 + cds TEV CLC
2358 2429 30556 30627 + cds BLRP CLC
3813 3846 30635 30668 + misc_recomb FRT CLC
dmel-5.43-2L
13447538 13447669 30745 30876 + three_prime_utr coding_transcript
13447538 13447669 30745 30876 + three_prime_utr coding_transcript
13444097 13445649 32765 34317 + gene CG9267 coding_transcript
13435637 13440955 37459 42777 - gene Tehao coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Hsp60D[29679]::S000169_fly_pretag_rev
TTATCATTACATAAAATGTTGTGTTTCTTCAAAACGCACTACCTAACCTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Hsp60D[29679]::S000169_fly_pretag_fwd
AGATTCCGAAAAATCAGGTCACCAAGGATCTTGCTAAACTTGTTGGAATGgaagtgcataccaatcaggacccgc
fasta Fasta