Clone: 2081450050271747 A12
Tagged gene: Buffy
Identifier: FBgn0040491
| Clone | FlyFos023707(pRedFlp-Hgr)(Buffy[18081]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation(click to hide details)
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good |
Genes in pFlyFos vector(click to hide details)
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-2R | ||||||||
| 7578801 | 7582958 | 11183 | 15340 | - | gene | CG13197 | coding_transcript | |
| 7576432 | 7578380 | 15761 | 17709 | + | gene | wal | coding_transcript | |
| 7566978 | 7576264 | 17877 | 28225 | - | gene | Buffy | coding_transcript | |
| 7566978 | 7576264 | 17877 | 28225 | - | mrna | FBtr0088077 | coding_transcript | |
| 7575895 | 7576264 | 17877 | 19308 | - | exon | coding_transcript | ||
| 7576063 | 7576264 | 17877 | 18078 | - | three_prime_utr | coding_transcript | ||
| 7575895 | 7576062 | 18079 | 19308 | - | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 2437 | 2470 | 18155 | 18188 | - | misc_recomb | FRT | CLC | |
| 2358 | 2429 | 18196 | 18267 | - | cds | BLRP | CLC | |
| 2337 | 2357 | 18268 | 18288 | - | cds | TEV | CLC | |
| 2313 | 2336 | 18289 | 18312 | - | cds | Precision cut site | CLC | |
| 2271 | 2312 | 18313 | 18354 | - | cds | V5 | CLC | |
| 1548 | 2264 | 18361 | 19077 | - | cds | SGFP | CLC | |
| 1482 | 1541 | 19084 | 19143 | - | cds | 2xTY1 | CLC | |
| dmel-5.43-2R | ||||||||
| 7575834 | 7575894 | 19309 | 19369 | - | intron | coding_transcript | ||
| 7575525 | 7575833 | 19370 | 19678 | - | exon | coding_transcript | ||
| 7575525 | 7575833 | 19370 | 19678 | - | cds | coding_transcript | ||
| 7575465 | 7575524 | 19679 | 19738 | - | intron | coding_transcript | ||
| 7575318 | 7575464 | 19739 | 19885 | - | exon | coding_transcript | ||
| 7575318 | 7575464 | 19739 | 19885 | - | cds | coding_transcript | ||
| 7575242 | 7575317 | 19886 | 19961 | - | intron | coding_transcript | ||
| 7574923 | 7575241 | 19962 | 20280 | - | exon | coding_transcript | ||
| 7574966 | 7575241 | 19962 | 20237 | - | cds | coding_transcript | ||
| 7574923 | 7574965 | 20238 | 20280 | - | five_prime_utr | coding_transcript | ||
| 7567092 | 7574922 | 20281 | 28111 | - | intron | coding_transcript | ||
| 7571654 | 7573937 | 21266 | 23549 | + | gene | CG13196 | coding_transcript | |
| 7571654 | 7573937 | 21266 | 23549 | + | mrna | FBtr0088084 | coding_transcript | |
| 7573564 | 7573937 | 21266 | 21639 | + | exon | coding_transcript | ||
| 7573920 | 7573937 | 21266 | 21283 | + | five_prime_utr | coding_transcript | ||
| 7573564 | 7573919 | 21284 | 21639 | + | cds | coding_transcript | ||
| 7573448 | 7573563 | 21640 | 21755 | + | intron | coding_transcript | ||
| 7572845 | 7573447 | 21756 | 22358 | + | exon | coding_transcript | ||
| 7572845 | 7573447 | 21756 | 22358 | + | cds | coding_transcript | ||
| 7572785 | 7572844 | 22359 | 22418 | + | intron | coding_transcript | ||
| 7572612 | 7572784 | 22419 | 22591 | + | exon | coding_transcript | ||
| 7572612 | 7572784 | 22419 | 22591 | + | cds | coding_transcript | ||
| 7572551 | 7572611 | 22592 | 22652 | + | intron | coding_transcript | ||
| 7571654 | 7572550 | 22653 | 23549 | + | exon | coding_transcript | ||
| 7572015 | 7572550 | 22653 | 23188 | + | cds | coding_transcript | ||
| 7571654 | 7572014 | 23189 | 23549 | + | three_prime_utr | coding_transcript | ||
| 7566978 | 7567091 | 28112 | 28225 | - | exon | coding_transcript | ||
| 7566978 | 7567091 | 28112 | 28225 | - | five_prime_utr | coding_transcript | ||
| 7563465 | 7565519 | 29684 | 31738 | - | gene | Tret1-2 | coding_transcript | |
| 7553259 | 7562287 | 32916 | 41944 | - | gene | Tret1-1 | coding_transcript | |
Sets(click to hide details)
Oligos(click to hide details)
| Name/Sequence | Export |
|---|---|
|
Buffy[18081]::S000169_fly_pretag_fwd
TTATATTTAACTTGATTGTACCAAAAATATACCAACGATTTACGAATTCCgaagtgcataccaatcaggacccgc
|
Fasta
|
|
Buffy[18081]::S000169_fly_pretag_rev
ATTATAGATCAAATCAATAGGATTTTTACGCTTAATAAACAAAATGTTTActtgtcgtcgtcatccttgtagtca
|
Fasta
|