Clone: 2081450050271747 F10

Tagged gene: Nnf1b
Identifier: FBgn0051658

Clone FlyFos023694(pRedFlp-Hgr)(Nnf1b[18549]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
818079 819520 10459 11900 + gene CG3876 coding_transcript
817046 818445 11534 12933 + gene CG15880 coding_transcript
816023 817032 12947 13956 - gene pex12 coding_transcript
813314 815951 14028 16665 + gene Saf6 coding_transcript
811609 813380 16599 18370 - gene KH1 coding_transcript
810586 811531 18448 20455 - gene Nnf1b coding_transcript
810586 811531 18448 20455 - mrna FBtr0078008 coding_transcript
811259 811531 18448 19782 - exon coding_transcript
811433 811531 18448 18546 - three_prime_utr coding_transcript
811259 811432 18547 19782 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 18623 18656 - misc_recomb FRT CLC
2358 2429 18664 18735 - cds BLRP CLC
2337 2357 18736 18756 - cds TEV CLC
2313 2336 18757 18780 - cds Precision cut site CLC
2271 2312 18781 18822 - cds V5 CLC
1548 2264 18829 19545 - cds SGFP CLC
1482 1541 19552 19611 - cds 2xTY1 CLC
dmel-5.43-2L
811204 811258 19783 19837 - intron coding_transcript
811089 811203 19838 19952 - exon coding_transcript
811089 811203 19838 19952 - cds coding_transcript
811022 811088 19953 20019 - intron coding_transcript
810809 811021 20020 20232 - exon coding_transcript
810809 811021 20020 20232 - cds coding_transcript
810759 810808 20233 20282 - intron coding_transcript
810586 810758 20283 20455 - exon coding_transcript
810646 810758 20283 20395 - cds coding_transcript
810586 810645 20396 20455 - five_prime_utr coding_transcript
786153 790795 40246 44888 + gene Pkg21D coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Nnf1b[18549]::S000169_fly_pretag_fwd
TAATGGAAATGGAACGGCTAATAAATGATTCGTATGTGCAGGAAGAAATGgaagtgcataccaatcaggacccgc
 fasta Fasta
Nnf1b[18549]::S000169_fly_pretag_rev
ATAATAATAATAATAATAATAATAATAATCTGTTTTTTTTTATAAAATTActtgtcgtcgtcatccttgtagtca
 fasta Fasta