Clone: 2081450050271747 F08

Tagged gene: ImpL2
Identifier: FBgn0001257

Clone FlyFos023640(pRedFlp-Hgr)(ImpL2[17319]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
4224699 4236147 17179 29689 - gene ImpL2 coding_transcript
4224699 4236147 17179 29689 - mrna FBtr0073248 coding_transcript
4224699 4227270 17179 20812 - mrna FBtr0073249 coding_transcript
4224699 4225950 17179 19492 - mrna FBtr0073250 coding_transcript
4224699 4224851 17179 18393 - exon coding_transcript
4224699 4224851 17179 18393 - exon coding_transcript
4224699 4224851 17179 18393 - exon coding_transcript
4224699 4224836 17179 17316 - three_prime_utr coding_transcript
4224699 4224836 17179 17316 - three_prime_utr coding_transcript
4224699 4224836 17179 17316 - three_prime_utr coding_transcript
4224837 4224851 17317 18393 - cds coding_transcript
4224837 4224851 17317 18393 - cds coding_transcript
4224837 4224851 17317 18393 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 17393 17426 - misc_recomb FRT CLC
2358 2429 17434 17505 - cds BLRP CLC
2337 2357 17506 17526 - cds TEV CLC
2313 2336 17527 17550 - cds Precision cut site CLC
2271 2312 17551 17592 - cds V5 CLC
1548 2264 17599 18315 - cds SGFP CLC
1482 1541 18322 18381 - cds 2xTY1 CLC
dmel-5.43-3L
4224852 4224926 18394 18468 - intron coding_transcript
4224852 4224926 18394 18468 - intron coding_transcript
4224852 4224926 18394 18468 - intron coding_transcript
4224927 4225706 18469 19248 - exon coding_transcript
4224927 4225706 18469 19248 - cds coding_transcript
4224927 4225706 18469 19248 - exon coding_transcript
4224927 4225706 18469 19248 - exon coding_transcript
4224927 4225706 18469 19248 - cds coding_transcript
4224927 4225703 18469 19245 - cds coding_transcript
4225704 4225706 19246 19248 - five_prime_utr coding_transcript
4225707 4235902 19249 29444 - intron coding_transcript
4225707 4226573 19249 20115 - intron coding_transcript
4225707 4225883 19249 19425 - intron coding_transcript
4225884 4225950 19426 19492 - exon coding_transcript
4225884 4225892 19426 19434 - cds coding_transcript
4225893 4225950 19435 19492 - five_prime_utr coding_transcript
4226574 4227270 20116 20812 - exon coding_transcript
4226574 4227270 20116 20812 - five_prime_utr coding_transcript
4235903 4236147 29445 29689 - exon coding_transcript
4235903 4235908 29445 29450 - cds coding_transcript
4235909 4236147 29451 29689 - five_prime_utr coding_transcript
4240173 4242603 33715 36145 + gene CG14997 coding_transcript
4242736 4246236 36278 39778 + gene pav coding_transcript
4246326 4254309 39868 47851 - gene ago coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
ImpL2[17319]::S000169_fly_pretag_fwd
TCCTAAGTAATCCCTACGTATTTTTCTTGTCCTTGCAGAATGAGGAAGACgaagtgcataccaatcaggacccgc
fasta Fasta
ImpL2[17319]::S000169_fly_pretag_rev
CGTCGTCAATCCAATAGCCTTTTGTTTTTTATTTGGTGGTAGGATCTTTActtgtcgtcgtcatccttgtagtca
fasta Fasta