Clone: 2081450050271747 G06

Tagged gene: Nep4
Identifier: FBgn0038818

Clone FlyFos023605(pRedFlp-Hgr)(Nep4[18854]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
16469876 16474436 9767 14327 - gene GluRIIE coding_transcript
16474311 16478172 14202 18063 - gene KaiRIA coding_transcript
16478289 16485328 18180 26281 - gene Nep4 coding_transcript
16478289 16485328 18180 26281 - mrna FBtr0083936 coding_transcript
16478289 16482562 18180 23515 - mrna FBtr0083937 coding_transcript
16478289 16478506 18180 18397 - exon coding_transcript
16478289 16478506 18180 18397 - three_prime_utr coding_transcript
16478289 16478506 18180 18397 - exon coding_transcript
16478289 16478506 18180 18397 - three_prime_utr coding_transcript
16478507 16478872 18398 18763 - intron coding_transcript
16478507 16478872 18398 18763 - intron coding_transcript
16478873 16479286 18764 20239 - exon coding_transcript
16478873 16479286 18764 20239 - exon coding_transcript
16478873 16478960 18764 18851 - three_prime_utr coding_transcript
16478873 16478960 18764 18851 - three_prime_utr coding_transcript
16478961 16479286 18852 20239 - cds coding_transcript
16478961 16479286 18852 20239 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 18928 18961 - misc_recomb FRT CLC
2358 2429 18969 19040 - cds BLRP CLC
2337 2357 19041 19061 - cds TEV CLC
2313 2336 19062 19085 - cds Precision cut site CLC
2271 2312 19086 19127 - cds V5 CLC
1548 2264 19134 19850 - cds SGFP CLC
1482 1541 19857 19916 - cds 2xTY1 CLC
dmel-5.43-3R
16479287 16479448 20240 20401 - intron coding_transcript
16479287 16479448 20240 20401 - intron coding_transcript
16479449 16480457 20402 21410 - exon coding_transcript
16479449 16480457 20402 21410 - cds coding_transcript
16479449 16480457 20402 21410 - exon coding_transcript
16479449 16480457 20402 21410 - cds coding_transcript
16480458 16480573 21411 21526 - intron coding_transcript
16480458 16480573 21411 21526 - intron coding_transcript
16480574 16482562 21527 23515 - exon coding_transcript
16480574 16482361 21527 23314 - cds coding_transcript
16480574 16482174 21527 23127 - exon coding_transcript
16480574 16482174 21527 23127 - cds coding_transcript
16482175 16485239 23128 26192 - intron coding_transcript
16482362 16482562 23315 23515 - five_prime_utr coding_transcript
16485240 16485328 26193 26281 - exon coding_transcript
16485240 16485240 26193 26193 - cds coding_transcript
16485241 16485328 26194 26281 - five_prime_utr coding_transcript
16495293 16498538 36246 39491 + gene Cpr92F coding_transcript
16501946 16503074 42899 44027 - gene CG4000 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Nep4[18854]::S000169_fly_pretag_fwd
ATTGTCCCCTCGGCTCACCGATGAACCCTCAGAAAAAGTGCAGCGTTTGGgaagtgcataccaatcaggacccgc
 fasta Fasta
Nep4[18854]::S000169_fly_pretag_rev
ATATGTCATGCTTAATACTCTTAGGCTATTGATTTGGTTCCCTCCGACTActtgtcgtcgtcatccttgtagtca
 fasta Fasta