Clone: 6185885656382698 G12

Tagged gene: MED27
Identifier: FBgn0037359

Clone FlyFos023522(pRedFlp-Hgr)(MED27[20700]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
1486359 1491168 11457 16266 - gene CG1109 coding_transcript
1481952 1485631 16994 20673 + gene CG2182 coding_transcript
1480867 1482058 20567 22820 - gene MED27 coding_transcript
1480867 1482058 20567 22820 - mrna FBtr0078712 coding_transcript
1481483 1482058 20567 22204 - exon coding_transcript
1481928 1482058 20567 20697 - three_prime_utr coding_transcript
1481483 1481927 20698 22204 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 20774 20807 - misc_recomb FRT CLC
2358 2429 20815 20886 - cds BLRP CLC
2337 2357 20887 20907 - cds TEV CLC
2313 2336 20908 20931 - cds Precision cut site CLC
2271 2312 20932 20973 - cds V5 CLC
1548 2264 20980 21696 - cds SGFP CLC
1482 1541 21703 21762 - cds 2xTY1 CLC
dmel-5.43-3R
1481423 1481482 22205 22264 - intron coding_transcript
1481156 1481422 22265 22531 - exon coding_transcript
1481156 1481422 22265 22531 - cds coding_transcript
1481098 1481155 22532 22589 - intron coding_transcript
1480867 1481097 22590 22820 - exon coding_transcript
1480928 1481097 22590 22759 - cds coding_transcript
1480867 1480927 22760 22820 - five_prime_utr coding_transcript
1478555 1480613 23074 25132 + gene elm coding_transcript
1474787 1478351 25336 28900 - gene sec23 coding_transcript
1464377 1474459 29228 39310 + gene MTA1-like coding_transcript
1462962 1464379 39308 40725 - gene CG12170 coding_transcript
1461623 1462567 41120 42064 + gene CG31548 coding_transcript
1460348 1461346 42341 43339 + gene CG31546 coding_transcript
1459346 1460362 43325 44341 - gene CG12171 coding_transcript
1457914 1459043 44644 45773 - gene CG31549 coding_transcript

Sets

Oligos

Name/Sequence Export
MED27[20700]::S000169_fly_pretag_fwd
GGCGGGACCTGCGCACTCTAGAACCCTTCCACGAGGACTGCCGAAATTGCgaagtgcataccaatcaggacccgc
 fasta Fasta
MED27[20700]::S000169_fly_pretag_rev
TGAAGTATACAAGTTACAACTATCGGCGTTAAATTACTTAAAAATCCTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta