Clone: 6185885656382698 D11

Tagged gene: CG32409
Identifier: FBgn0052409

Clone FlyFos023508(pRedFlp-Hgr)(CG32409[25219]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
5897307 5899799 19344 21836 + gene CG10486 coding_transcript
5895986 5897152 21991 23157 + gene CG32413 coding_transcript
5893935 5895550 23593 25208 + gene QC coding_transcript
5892642 5894036 25107 27563 - gene CG32409 coding_transcript
5892642 5894036 25107 27563 - mrna FBtr0077030 coding_transcript
5893773 5894036 25107 26432 - exon coding_transcript
5893927 5894036 25107 25216 - three_prime_utr coding_transcript
5893935 5894017 25126 25208 + three_prime_utr coding_transcript
5893773 5893926 25217 26432 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 25293 25326 - misc_recomb FRT CLC
2358 2429 25334 25405 - cds BLRP CLC
2337 2357 25406 25426 - cds TEV CLC
2313 2336 25427 25450 - cds Precision cut site CLC
2271 2312 25451 25492 - cds V5 CLC
1548 2264 25499 26215 - cds SGFP CLC
1482 1541 26222 26281 - cds 2xTY1 CLC
dmel-5.43-3L
5893709 5893772 26433 26496 - intron coding_transcript
5893215 5893708 26497 26990 - exon coding_transcript
5893215 5893708 26497 26990 - cds coding_transcript
5893158 5893214 26991 27047 - intron coding_transcript
5892905 5893157 27048 27300 - exon coding_transcript
5892905 5893157 27048 27300 - cds coding_transcript
5892848 5892904 27301 27357 - intron coding_transcript
5892642 5892847 27358 27563 - exon coding_transcript
5892699 5892847 27358 27506 - cds coding_transcript
5892642 5892698 27507 27563 - five_prime_utr coding_transcript
5890738 5892495 27710 29467 - gene CG5592 coding_transcript
5888785 5890479 29726 31420 + gene Pole2 coding_transcript
5875507 5876892 43313 44698 - gene CG13287 coding_transcript

Sets

Oligos

Name/Sequence Export
CG32409[25219]::S000169_fly_pretag_rev
AAGTGTTTTTCGTTAATTAAAGTGTCCTAAGGCATGTGTAATTCTTCCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
CG32409[25219]::S000169_fly_pretag_fwd
GTGGTCAAAGGGCTCCGGGCAAGAAGGCGCAAGCCGGTCGCAAACGTCGCgaagtgcataccaatcaggacccgc
 fasta Fasta