Clone: 6185885656382698 C01
Tagged gene: Pcf11
Identifier: FBgn0022063
| Clone | FlyFos023334(pRedFlp-Hgr)(Pcf11[37363]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | bad |
Genes in pFlyFos vector
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-2R | ||||||||
| 10774676 | 10776514 | 9739 | 11577 | + | gene | Cyp6a8 | coding_transcript | |
| 10772769 | 10774456 | 11797 | 13484 | + | gene | Cyp6a21 | coding_transcript | |
| 10769625 | 10771551 | 14702 | 16628 | - | gene | Cyp6a20 | coding_transcript | |
| 10766861 | 10768877 | 17376 | 19392 | + | gene | Cyp6a9 | coding_transcript | |
| 10765293 | 10767257 | 18996 | 20960 | - | gene | Cyp6a19 | coding_transcript | |
| 10763338 | 10765159 | 21094 | 22915 | - | gene | Cyp6a23 | coding_transcript | |
| 10761459 | 10763063 | 23190 | 24794 | - | gene | Cyp6a17 | coding_transcript | |
| 10759178 | 10760970 | 25283 | 27075 | - | gene | Cyp6a22 | coding_transcript | |
| 10748661 | 10758575 | 27678 | 38654 | + | gene | Pcf11 | coding_transcript | |
| 10748661 | 10758575 | 27678 | 38654 | + | mrna | FBtr0302294 | coding_transcript | |
| 10748661 | 10758575 | 27678 | 38654 | + | mrna | FBtr0302560 | coding_transcript | |
| 10754118 | 10758575 | 27678 | 32135 | + | mrna | FBtr0301513 | coding_transcript | |
| 10757151 | 10758575 | 27678 | 29102 | + | mrna | FBtr0302109 | coding_transcript | |
| 10758425 | 10758575 | 27678 | 27828 | + | exon | coding_transcript | ||
| 10758425 | 10758575 | 27678 | 27828 | + | five_prime_utr | coding_transcript | ||
| 10758425 | 10758575 | 27678 | 27828 | + | exon | coding_transcript | ||
| 10758425 | 10758575 | 27678 | 27828 | + | five_prime_utr | coding_transcript | ||
| 10758425 | 10758575 | 27678 | 27828 | + | exon | coding_transcript | ||
| 10758425 | 10758575 | 27678 | 27828 | + | five_prime_utr | coding_transcript | ||
| 10758425 | 10758575 | 27678 | 27828 | + | exon | coding_transcript | ||
| 10758425 | 10758575 | 27678 | 27828 | + | five_prime_utr | coding_transcript | ||
| 10757818 | 10758424 | 27829 | 28435 | + | intron | coding_transcript | ||
| 10757818 | 10758424 | 27829 | 28435 | + | intron | coding_transcript | ||
| 10757818 | 10758424 | 27829 | 28435 | + | intron | coding_transcript | ||
| 10757818 | 10758424 | 27829 | 28435 | + | intron | coding_transcript | ||
| 10757151 | 10757817 | 28436 | 29102 | + | exon | coding_transcript | ||
| 10757554 | 10757817 | 28436 | 28699 | + | exon | coding_transcript | ||
| 10757554 | 10757817 | 28436 | 28699 | + | exon | coding_transcript | ||
| 10757554 | 10757817 | 28436 | 28699 | + | exon | coding_transcript | ||
| 10757737 | 10757817 | 28436 | 28516 | + | five_prime_utr | coding_transcript | ||
| 10757737 | 10757817 | 28436 | 28516 | + | five_prime_utr | coding_transcript | ||
| 10757737 | 10757817 | 28436 | 28516 | + | five_prime_utr | coding_transcript | ||
| 10757737 | 10757817 | 28436 | 28516 | + | five_prime_utr | coding_transcript | ||
| 10757476 | 10757736 | 28517 | 28777 | + | cds | coding_transcript | ||
| 10757554 | 10757736 | 28517 | 28699 | + | cds | coding_transcript | ||
| 10757554 | 10757736 | 28517 | 28699 | + | cds | coding_transcript | ||
| 10757554 | 10757736 | 28517 | 28699 | + | cds | coding_transcript | ||
| 10756538 | 10757553 | 28700 | 29715 | + | intron | coding_transcript | ||
| 10756538 | 10757553 | 28700 | 29715 | + | intron | coding_transcript | ||
| 10756538 | 10757553 | 28700 | 29715 | + | intron | coding_transcript | ||
| 10757151 | 10757475 | 28778 | 29102 | + | three_prime_utr | coding_transcript | ||
| 10756412 | 10756537 | 29716 | 29841 | + | exon | coding_transcript | ||
| 10756412 | 10756537 | 29716 | 29841 | + | cds | coding_transcript | ||
| 10756412 | 10756537 | 29716 | 29841 | + | exon | coding_transcript | ||
| 10756412 | 10756537 | 29716 | 29841 | + | cds | coding_transcript | ||
| 10756412 | 10756537 | 29716 | 29841 | + | exon | coding_transcript | ||
| 10756412 | 10756537 | 29716 | 29841 | + | cds | coding_transcript | ||
| 10756349 | 10756411 | 29842 | 29904 | + | intron | coding_transcript | ||
| 10756349 | 10756411 | 29842 | 29904 | + | intron | coding_transcript | ||
| 10756349 | 10756411 | 29842 | 29904 | + | intron | coding_transcript | ||
| 10756133 | 10756348 | 29905 | 30120 | + | exon | coding_transcript | ||
| 10756133 | 10756348 | 29905 | 30120 | + | cds | coding_transcript | ||
| 10756133 | 10756348 | 29905 | 30120 | + | exon | coding_transcript | ||
| 10756133 | 10756348 | 29905 | 30120 | + | cds | coding_transcript | ||
| 10756133 | 10756348 | 29905 | 30120 | + | exon | coding_transcript | ||
| 10756133 | 10756348 | 29905 | 30120 | + | cds | coding_transcript | ||
| 10756068 | 10756132 | 30121 | 30185 | + | intron | coding_transcript | ||
| 10756068 | 10756132 | 30121 | 30185 | + | intron | coding_transcript | ||
| 10756068 | 10756132 | 30121 | 30185 | + | intron | coding_transcript | ||
| 10755738 | 10756067 | 30186 | 30515 | + | exon | coding_transcript | ||
| 10755738 | 10756067 | 30186 | 30515 | + | cds | coding_transcript | ||
| 10755738 | 10756067 | 30186 | 30515 | + | exon | coding_transcript | ||
| 10755738 | 10756067 | 30186 | 30515 | + | cds | coding_transcript | ||
| 10755738 | 10756067 | 30186 | 30515 | + | exon | coding_transcript | ||
| 10755738 | 10756067 | 30186 | 30515 | + | cds | coding_transcript | ||
| 10755677 | 10755737 | 30516 | 30576 | + | intron | coding_transcript | ||
| 10755677 | 10755737 | 30516 | 30576 | + | intron | coding_transcript | ||
| 10755677 | 10755737 | 30516 | 30576 | + | intron | coding_transcript | ||
| 10754118 | 10755676 | 30577 | 32135 | + | exon | coding_transcript | ||
| 10754810 | 10755676 | 30577 | 31443 | + | cds | coding_transcript | ||
| 10754989 | 10755676 | 30577 | 31264 | + | exon | coding_transcript | ||
| 10754989 | 10755676 | 30577 | 31264 | + | cds | coding_transcript | ||
| 10754989 | 10755676 | 30577 | 31264 | + | exon | coding_transcript | ||
| 10754989 | 10755676 | 30577 | 31264 | + | cds | coding_transcript | ||
| 10753239 | 10754988 | 31265 | 33014 | + | intron | coding_transcript | ||
| 10753581 | 10754988 | 31265 | 32672 | + | intron | coding_transcript | ||
| 10754118 | 10754809 | 31444 | 32135 | + | three_prime_utr | coding_transcript | ||
| 10753013 | 10753580 | 32673 | 33240 | + | exon | coding_transcript | ||
| 10753013 | 10753580 | 32673 | 33240 | + | cds | coding_transcript | ||
| 10753013 | 10753238 | 33015 | 33240 | + | exon | coding_transcript | ||
| 10753013 | 10753238 | 33015 | 33240 | + | cds | coding_transcript | ||
| 10752953 | 10753012 | 33241 | 33300 | + | intron | coding_transcript | ||
| 10752953 | 10753012 | 33241 | 33300 | + | intron | coding_transcript | ||
| 10752847 | 10752952 | 33301 | 33406 | + | exon | coding_transcript | ||
| 10752847 | 10752952 | 33301 | 33406 | + | cds | coding_transcript | ||
| 10752847 | 10752952 | 33301 | 33406 | + | exon | coding_transcript | ||
| 10752847 | 10752952 | 33301 | 33406 | + | cds | coding_transcript | ||
| 10752780 | 10752846 | 33407 | 33473 | + | intron | coding_transcript | ||
| 10752780 | 10752846 | 33407 | 33473 | + | intron | coding_transcript | ||
| 10752677 | 10752779 | 33474 | 33576 | + | exon | coding_transcript | ||
| 10752677 | 10752779 | 33474 | 33576 | + | cds | coding_transcript | ||
| 10752677 | 10752779 | 33474 | 33576 | + | exon | coding_transcript | ||
| 10752677 | 10752779 | 33474 | 33576 | + | cds | coding_transcript | ||
| 10752564 | 10752676 | 33577 | 33689 | + | intron | coding_transcript | ||
| 10752564 | 10752676 | 33577 | 33689 | + | intron | coding_transcript | ||
| 10750236 | 10752563 | 33690 | 36017 | + | exon | coding_transcript | ||
| 10750236 | 10752563 | 33690 | 36017 | + | cds | coding_transcript | ||
| 10750236 | 10752563 | 33690 | 36017 | + | exon | coding_transcript | ||
| 10750236 | 10752563 | 33690 | 36017 | + | cds | coding_transcript | ||
| 10750174 | 10750235 | 36018 | 36079 | + | intron | coding_transcript | ||
| 10750174 | 10750235 | 36018 | 36079 | + | intron | coding_transcript | ||
| 10749700 | 10750173 | 36080 | 36553 | + | exon | coding_transcript | ||
| 10749700 | 10750173 | 36080 | 36553 | + | cds | coding_transcript | ||
| 10749700 | 10750173 | 36080 | 36553 | + | exon | coding_transcript | ||
| 10749700 | 10750173 | 36080 | 36553 | + | cds | coding_transcript | ||
| 10749627 | 10749699 | 36554 | 36626 | + | intron | coding_transcript | ||
| 10749627 | 10749699 | 36554 | 36626 | + | intron | coding_transcript | ||
| 10748661 | 10749626 | 36627 | 38654 | + | exon | coding_transcript | ||
| 10748661 | 10749626 | 36627 | 38654 | + | exon | coding_transcript | ||
| 10748887 | 10749626 | 36627 | 38428 | + | cds | coding_transcript | ||
| 10748887 | 10749626 | 36627 | 38428 | + | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 1482 | 1541 | 37364 | 37423 | + | cds | 2xTY1 | CLC | |
| 1548 | 2264 | 37430 | 38146 | + | cds | SGFP | CLC | |
| 2271 | 2312 | 38153 | 38194 | + | cds | V5 | CLC | |
| 2313 | 2336 | 38195 | 38218 | + | cds | Precision cut site | CLC | |
| 2337 | 2357 | 38219 | 38239 | + | cds | TEV | CLC | |
| 2358 | 2429 | 38240 | 38311 | + | cds | BLRP | CLC | |
| 3813 | 3846 | 38319 | 38352 | + | misc_recomb | FRT | CLC | |
| dmel-5.43-2R | ||||||||
| 10748661 | 10748886 | 38429 | 38654 | + | three_prime_utr | coding_transcript | ||
| 10748661 | 10748886 | 38429 | 38654 | + | three_prime_utr | coding_transcript | ||
| 10746350 | 10748250 | 39065 | 40965 | + | gene | U3-55K | coding_transcript | |
| 10744952 | 10746138 | 41177 | 42363 | + | gene | CG33506 | coding_transcript | |
| 10742947 | 10745007 | 42308 | 44368 | - | gene | HPS1 | coding_transcript | |
Sets
Oligos
| Name/Sequence | Export |
|---|---|
|
Pcf11[37363]::S000169_fly_pretag_fwd
CGAAAAAGGTGCAGAACGGCATCGAAACGTCGGGCCTGTGCTCGATCATGgaagtgcataccaatcaggacccgc
|
Fasta
|
|
Pcf11[37363]::S000169_fly_pretag_rev
GTATTAGGAATATCGCCAAGTCCCTCCACTCAGACGGCCGATTTGATTTActtgtcgtcgtcatccttgtagtca
|
Fasta
|