Clone: 2086307455001194 D12

Tagged gene: GstE10
Identifier: FBgn0063499

Clone FlyFos023256(pRedFlp-Hgr)(GstE10[21419]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
14274102 14274535 11271 11704 + gene IM2 coding_transcript
14275625 14275995 12794 13164 + gene IM3 coding_transcript
14276600 14276963 13769 14132 + gene CG16836 coding_transcript
14277463 14277752 14632 14921 + gene CG15065 coding_transcript
14277847 14278114 15016 15283 - gene CG15068 coding_transcript
14281170 14282787 18339 19956 + gene Idgf5 coding_transcript
14284143 14285031 21312 23262 - gene GstE10 coding_transcript
14284143 14285031 21312 23262 - mrna FBtr0086726 coding_transcript
14284143 14285031 21312 23262 - exon coding_transcript
14284143 14284247 21312 21416 - three_prime_utr coding_transcript
14284248 14284970 21417 23201 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 21493 21526 - misc_recomb FRT CLC
2358 2429 21534 21605 - cds BLRP CLC
2337 2357 21606 21626 - cds TEV CLC
2313 2336 21627 21650 - cds Precision cut site CLC
2271 2312 21651 21692 - cds V5 CLC
1548 2264 21699 22415 - cds SGFP CLC
1482 1541 22422 22481 - cds 2xTY1 CLC
dmel-5.43-2R
14284971 14285031 23202 23262 - five_prime_utr coding_transcript
14285898 14286728 24129 24959 + gene GstE1 coding_transcript
14286998 14287880 25229 26111 + gene GstE2 coding_transcript
14288222 14288972 26453 27203 + gene GstE3 coding_transcript
14290771 14291591 29002 29822 + gene GstE4 coding_transcript
14292125 14292843 30356 31074 + gene GstE5 coding_transcript
14293518 14294266 31749 32497 + gene GstE6 coding_transcript
14294425 14295193 32656 33424 + gene GstE7 coding_transcript
14295339 14296067 33570 34298 + gene GstE8 coding_transcript
14296516 14297340 34747 35571 + gene GstE9 coding_transcript
14297296 14299024 35527 37255 - gene imd coding_transcript
14299511 14308629 37742 46860 + gene Dp1 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
GstE10[21419]::S000169_fly_pretag_rev
TTTACTTTGGGCAATGCTCTCTTTTCGATGCGACAATGGCTTCCGCATCActtgtcgtcgtcatccttgtagtca
 fasta Fasta
GstE10[21419]::S000169_fly_pretag_fwd
AGCTGTGGCAAAAGGCCTTTGAGGACATCAAGAGCGGAGCTGGAAAACAGgaagtgcataccaatcaggacccgc
 fasta Fasta