Clone: 2086307455001194 C12

Tagged gene: GstE4
Identifier: FBgn0063496

Clone FlyFos023256(pRedFlp-Hgr)(GstE4[28614]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
14274102 14274535 11271 11704 + gene IM2 coding_transcript
14275625 14275995 12794 13164 + gene IM3 coding_transcript
14276600 14276963 13769 14132 + gene CG16836 coding_transcript
14277463 14277752 14632 14921 + gene CG15065 coding_transcript
14277847 14278114 15016 15283 - gene CG15068 coding_transcript
14281170 14282787 18339 19956 + gene Idgf5 coding_transcript
14284143 14285031 21312 22200 - gene GstE10 coding_transcript
14285898 14286728 23067 23897 + gene GstE1 coding_transcript
14286998 14287880 24167 25049 + gene GstE2 coding_transcript
14288222 14288972 25391 26141 + gene GstE3 coding_transcript
14290771 14291591 27940 29822 + gene GstE4 coding_transcript
14290771 14291591 27940 29822 + mrna FBtr0086672 coding_transcript
14290771 14291591 27940 29822 + exon coding_transcript
14290771 14290779 27940 27948 + five_prime_utr coding_transcript
14290780 14291448 27949 29679 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 28615 28674 + cds 2xTY1 CLC
1548 2264 28681 29397 + cds SGFP CLC
2271 2312 29404 29445 + cds V5 CLC
2313 2336 29446 29469 + cds Precision cut site CLC
2337 2357 29470 29490 + cds TEV CLC
2358 2429 29491 29562 + cds BLRP CLC
3813 3846 29570 29603 + misc_recomb FRT CLC
dmel-5.43-2R
14291449 14291591 29680 29822 + three_prime_utr coding_transcript
14292125 14292843 30356 31074 + gene GstE5 coding_transcript
14293518 14294266 31749 32497 + gene GstE6 coding_transcript
14294425 14295193 32656 33424 + gene GstE7 coding_transcript
14295339 14296067 33570 34298 + gene GstE8 coding_transcript
14296516 14297340 34747 35571 + gene GstE9 coding_transcript
14297296 14299024 35527 37255 - gene imd coding_transcript
14299511 14308629 37742 46860 + gene Dp1 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
GstE4[28614]::S000169_fly_pretag_rev
TCCAATTAAAATGTTAAGCCATTTACTGTCGAAATCAAGACTAGTGCTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
GstE4[28614]::S000169_fly_pretag_fwd
CTGCCCAGTTCGTGGAGCTCTTGAGGTCCAAAAACTTCACCATAGTTTCGgaagtgcataccaatcaggacccgc
 fasta Fasta