Clone: 2086307455001194 B07

Tagged gene: Ucp4B
Identifier: FBgn0031758

Clone FlyFos023176(pRedFlp-Hgr)(Ucp4B[31676]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
5949484 5955238 10179 15933 + gene CG9044 coding_transcript
5955312 5956466 16007 17161 - gene CG13999 coding_transcript
5956512 5956805 17207 17500 - gene CG13998 coding_transcript
5957004 5957628 17699 18323 + gene Vm26Ab coding_transcript
5958005 5958631 18700 19326 - gene Vm26Ac coding_transcript
5959700 5960340 20395 21035 - gene Vm26Aa coding_transcript
5963087 5964322 23782 25017 - gene psd coding_transcript
5965391 5967752 26086 28447 - gene CG13992 coding_transcript
5968566 5969761 29261 30456 + gene Ucp4C coding_transcript
5969871 5971025 30566 32782 + gene Ucp4B coding_transcript
5969871 5971025 30566 32782 + mrna FBtr0079173 coding_transcript
5969871 5971025 30566 32782 + mrna FBtr0309286 coding_transcript
5969871 5970651 30566 31346 + mrna FBtr0079174 coding_transcript
5969871 5970651 30566 31346 + exon coding_transcript
5969871 5970559 30566 31254 + exon coding_transcript
5969871 5970559 30566 31254 + exon coding_transcript
5969871 5969901 30566 30596 + five_prime_utr coding_transcript
5969871 5969901 30566 30596 + five_prime_utr coding_transcript
5969871 5969901 30566 30596 + five_prime_utr coding_transcript
5969902 5970570 30597 31265 + cds coding_transcript
5969902 5970559 30597 31254 + cds coding_transcript
5969902 5970559 30597 31254 + cds coding_transcript
5970560 5970638 31255 31333 + intron coding_transcript
5970560 5970628 31255 31323 + intron coding_transcript
5970571 5970651 31266 31346 + three_prime_utr coding_transcript
5970629 5971025 31324 32782 + exon coding_transcript
5970629 5970984 31324 32741 + cds coding_transcript
5970639 5971025 31334 32782 + exon coding_transcript
5970639 5970667 31334 31362 + cds coding_transcript
5970668 5971025 31363 32782 + three_prime_utr coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 31677 31736 + cds 2xTY1 CLC
1548 2264 31743 32459 + cds SGFP CLC
2271 2312 32466 32507 + cds V5 CLC
2313 2336 32508 32531 + cds Precision cut site CLC
2337 2357 32532 32552 + cds TEV CLC
2358 2429 32553 32624 + cds BLRP CLC
3813 3846 32632 32665 + misc_recomb FRT CLC
dmel-5.43-2L
5970985 5971025 32742 32782 + three_prime_utr coding_transcript
5972115 5981025 33872 42782 - gene chic coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Ucp4B[31676]::S000169_fly_pretag_fwd
TCTGGATGACCTTCGAGCAGATTAGACGGTTTCGTGGCAGCGAAGGTTACgaagtgcataccaatcaggacccgc
fasta Fasta
Ucp4B[31676]::S000169_fly_pretag_rev
GATATTAATGATATTTTACTCTGTTATATTTTAAACTGCATCTTTAGCTActtgtcgtcgtcatccttgtagtca
fasta Fasta