Clone: 2086307455001194 F04

Tagged gene: CG8235
Identifier: FBgn0033351

Clone FlyFos023159(pRedFlp-Hgr)(CG8235[22976]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
4802033 4805739 10430 14136 + gene lin coding_transcript
4805213 4806474 13610 14871 - gene CG34219 coding_transcript
4806445 4807871 14842 16268 - gene CG8248 coding_transcript
4807775 4810256 16172 18653 - gene CG8247 coding_transcript
4810293 4812851 18690 21248 + gene CG8243 coding_transcript
4813194 4814395 21591 22792 + gene CG8237 coding_transcript
4814519 4815605 22916 25064 - gene CG8235 coding_transcript
4814519 4815605 22916 25064 - mrna FBtr0088676 coding_transcript
4814519 4815389 22916 24848 - exon coding_transcript
4814519 4814576 22916 22973 - three_prime_utr coding_transcript
4814577 4815389 22974 24848 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 23050 23083 - misc_recomb FRT CLC
2358 2429 23091 23162 - cds BLRP CLC
2337 2357 23163 23183 - cds TEV CLC
2313 2336 23184 23207 - cds Precision cut site CLC
2271 2312 23208 23249 - cds V5 CLC
1548 2264 23256 23972 - cds SGFP CLC
1482 1541 23979 24038 - cds 2xTY1 CLC
dmel-5.43-2R
4815390 4815445 24849 24904 - intron coding_transcript
4815446 4815605 24905 25064 - exon coding_transcript
4815446 4815604 24905 25063 - cds coding_transcript
4815605 4815605 25064 25064 - five_prime_utr coding_transcript
4815679 4820608 25138 30067 + gene CG8232 coding_transcript
4820928 4821950 30387 31409 + gene CG13749 coding_transcript
4822078 4827299 31537 36758 - gene FANCI coding_transcript
4827920 4828641 37379 38100 + gene CG13748 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG8235[22976]::S000169_fly_pretag_fwd
CTGGCAAGGGCAACGTGGTGGCCCAAACCCTGAAGAACGTCAATGTGAAGgaagtgcataccaatcaggacccgc
 fasta Fasta
CG8235[22976]::S000169_fly_pretag_rev
GGTGATGTTTCGTTTATTGGCTCAAAGCTTTAGTTTCGTTTATTGGCTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta