Clone: 2086307455001194 D02
Tagged gene: Kaz1-ORFB
Identifier: FBgn0063923
| Clone | FlyFos023140(pRedFlp-Hgr)(Kaz1-ORFB[23660]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation(click to hide details)
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | bad |
Genes in pFlyFos vector(click to hide details)
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-3L | ||||||||
| 206134 | 207527 | 11905 | 13298 | + | gene | CG13876 | coding_transcript | |
| 207887 | 211462 | 13658 | 17233 | - | gene | CG7028 | coding_transcript | |
| 211636 | 212687 | 17407 | 18458 | - | gene | thoc7 | coding_transcript | |
| 212275 | 216479 | 18046 | 22250 | + | gene | CG16940 | coding_transcript | |
| 216645 | 218058 | 22416 | 24891 | + | gene | Kaz1-ORFB | coding_transcript | |
| 216645 | 218058 | 22416 | 24891 | + | mrna | FBtr0100851 | coding_transcript | |
| 216645 | 216706 | 22416 | 22477 | + | exon | coding_transcript | ||
| 216645 | 216706 | 22416 | 22477 | + | five_prime_utr | coding_transcript | ||
| 216692 | 218058 | 22463 | 24891 | + | mrna | FBtr0072493 | coding_transcript | |
| 216692 | 217109 | 22463 | 22880 | + | exon | coding_transcript | ||
| 216692 | 217109 | 22463 | 22880 | + | five_prime_utr | coding_transcript | ||
| 216707 | 216760 | 22478 | 22531 | + | intron | coding_transcript | ||
| 216761 | 217109 | 22532 | 22880 | + | exon | coding_transcript | ||
| 216761 | 217109 | 22532 | 22880 | + | five_prime_utr | coding_transcript | ||
| 217040 | 218058 | 22811 | 24891 | + | mrna | FBtr0072490 | coding_transcript | |
| 217040 | 217109 | 22811 | 22880 | + | exon | coding_transcript | ||
| 217040 | 217109 | 22811 | 22880 | + | five_prime_utr | coding_transcript | ||
| 217110 | 217219 | 22881 | 22990 | + | intron | coding_transcript | ||
| 217110 | 217219 | 22881 | 22990 | + | intron | coding_transcript | ||
| 217110 | 217152 | 22881 | 22923 | + | intron | coding_transcript | ||
| 217153 | 217775 | 22924 | 23546 | + | exon | coding_transcript | ||
| 217153 | 217519 | 22924 | 23290 | + | five_prime_utr | coding_transcript | ||
| 217220 | 217442 | 22991 | 23213 | + | exon | coding_transcript | ||
| 217220 | 217442 | 22991 | 23213 | + | exon | coding_transcript | ||
| 217220 | 217420 | 22991 | 23191 | + | five_prime_utr | coding_transcript | ||
| 217220 | 217420 | 22991 | 23191 | + | five_prime_utr | coding_transcript | ||
| 217385 | 218058 | 23156 | 24891 | + | mrna | FBtr0100224 | coding_transcript | |
| 217385 | 217442 | 23156 | 23213 | + | exon | coding_transcript | ||
| 217385 | 217420 | 23156 | 23191 | + | five_prime_utr | coding_transcript | ||
| 217390 | 218058 | 23161 | 24891 | + | mrna | FBtr0072488 | coding_transcript | |
| 217390 | 217442 | 23161 | 23213 | + | exon | coding_transcript | ||
| 217390 | 217420 | 23161 | 23191 | + | five_prime_utr | coding_transcript | ||
| 217421 | 217442 | 23192 | 23213 | + | cds | coding_transcript | ||
| 217421 | 217442 | 23192 | 23213 | + | cds | coding_transcript | ||
| 217421 | 217442 | 23192 | 23213 | + | cds | coding_transcript | ||
| 217421 | 217442 | 23192 | 23213 | + | cds | coding_transcript | ||
| 217443 | 217490 | 23214 | 23261 | + | intron | coding_transcript | ||
| 217443 | 217490 | 23214 | 23261 | + | intron | coding_transcript | ||
| 217443 | 217490 | 23214 | 23261 | + | intron | coding_transcript | ||
| 217443 | 217490 | 23214 | 23261 | + | intron | coding_transcript | ||
| 217491 | 218058 | 23262 | 24891 | + | exon | coding_transcript | ||
| 217491 | 217816 | 23262 | 23587 | + | cds | coding_transcript | ||
| 217491 | 217775 | 23262 | 23546 | + | exon | coding_transcript | ||
| 217491 | 217775 | 23262 | 23546 | + | cds | coding_transcript | ||
| 217491 | 217775 | 23262 | 23546 | + | exon | coding_transcript | ||
| 217491 | 217775 | 23262 | 23546 | + | cds | coding_transcript | ||
| 217491 | 217775 | 23262 | 23546 | + | exon | coding_transcript | ||
| 217491 | 217775 | 23262 | 23546 | + | cds | coding_transcript | ||
| 217520 | 217775 | 23291 | 23546 | + | cds | coding_transcript | ||
| 217776 | 217854 | 23547 | 23625 | + | intron | coding_transcript | ||
| 217776 | 217854 | 23547 | 23625 | + | intron | coding_transcript | ||
| 217776 | 217854 | 23547 | 23625 | + | intron | coding_transcript | ||
| 217776 | 217854 | 23547 | 23625 | + | intron | coding_transcript | ||
| 217817 | 218058 | 23588 | 24891 | + | three_prime_utr | coding_transcript | ||
| 217855 | 218058 | 23626 | 24891 | + | exon | coding_transcript | ||
| 217855 | 218058 | 23626 | 24891 | + | exon | coding_transcript | ||
| 217855 | 218058 | 23626 | 24891 | + | exon | coding_transcript | ||
| 217855 | 218058 | 23626 | 24891 | + | exon | coding_transcript | ||
| 217855 | 217892 | 23626 | 24725 | + | cds | coding_transcript | ||
| 217855 | 217892 | 23626 | 24725 | + | cds | coding_transcript | ||
| 217855 | 217892 | 23626 | 24725 | + | cds | coding_transcript | ||
| 217855 | 217892 | 23626 | 24725 | + | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 1482 | 1541 | 23661 | 23720 | + | cds | 2xTY1 | CLC | |
| 1548 | 2264 | 23727 | 24443 | + | cds | SGFP | CLC | |
| 2271 | 2312 | 24450 | 24491 | + | cds | V5 | CLC | |
| 2313 | 2336 | 24492 | 24515 | + | cds | Precision cut site | CLC | |
| 2337 | 2357 | 24516 | 24536 | + | cds | TEV | CLC | |
| 2358 | 2429 | 24537 | 24608 | + | cds | BLRP | CLC | |
| 3813 | 3846 | 24616 | 24649 | + | misc_recomb | FRT | CLC | |
| dmel-5.43-3L | ||||||||
| 217893 | 218058 | 24726 | 24891 | + | three_prime_utr | coding_transcript | ||
| 217893 | 218058 | 24726 | 24891 | + | three_prime_utr | coding_transcript | ||
| 217893 | 218058 | 24726 | 24891 | + | three_prime_utr | coding_transcript | ||
| 217893 | 218058 | 24726 | 24891 | + | three_prime_utr | coding_transcript | ||
| 217932 | 223069 | 24765 | 29902 | - | gene | pyx | coding_transcript | |
| 218067 | 218663 | 24900 | 25496 | + | gene | CG13877 | coding_transcript | |
| 224106 | 225605 | 30939 | 32438 | + | gene | CG33229 | coding_transcript | |
| 230971 | 231604 | 37804 | 38437 | - | gene | CG42846 | coding_transcript | |
| 231681 | 232350 | 38514 | 39183 | - | gene | CG34454 | coding_transcript | |
| 232480 | 233351 | 39313 | 40184 | - | gene | CG34453 | coding_transcript | |
Sets(click to hide details)
Oligos(click to hide details)
| Name/Sequence | Export |
|---|---|
|
Kaz1-ORFB[23660]::S000169_fly_pretag_fwd
CCTTCTTCATTATAGACATCCGGAAGGTGCACAAGGGAATTTGTCAGACTgaagtgcataccaatcaggacccgc
|
Fasta
|
|
Kaz1-ORFB[23660]::S000169_fly_pretag_rev
ACGTTCATGCTTACATACACAAGTGAACTGAATTCAAGCGATCTAGACTActtgtcgtcgtcatccttgtagtca
|
Fasta
|