Clone: 1367792136965469 E12

Tagged gene: eff
Identifier: FBgn0011217

Clone FlyFos023128(pRedFlp-Hgr)(eff[32783]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
10571623 10573043 18960 20380 + gene smp-30 coding_transcript
10558427 10566933 25070 34638 + gene eff coding_transcript
10558427 10566933 25070 34638 + mrna FBtr0083018 coding_transcript
10566729 10566933 25070 25274 + exon coding_transcript
10566729 10566933 25070 25274 + five_prime_utr coding_transcript
10566663 10566728 25275 25340 + intron coding_transcript
10566102 10566662 25341 25901 + exon coding_transcript
10566126 10566662 25341 25877 + five_prime_utr coding_transcript
10558427 10566486 25517 34638 + mrna FBtr0310554 coding_transcript
10566102 10566486 25517 25901 + exon coding_transcript
10566126 10566486 25517 25877 + five_prime_utr coding_transcript
10566102 10566125 25878 25901 + cds coding_transcript
10566102 10566125 25878 25901 + cds coding_transcript
10563206 10566101 25902 28797 + intron coding_transcript
10563206 10566101 25902 28797 + intron coding_transcript
10563142 10563205 28798 28861 + exon coding_transcript
10563142 10563205 28798 28861 + cds coding_transcript
10563142 10563205 28798 28861 + exon coding_transcript
10563142 10563205 28798 28861 + cds coding_transcript
10560141 10563141 28862 31862 + intron coding_transcript
10560141 10563141 28862 31862 + intron coding_transcript
10560109 10560140 31863 31894 + exon coding_transcript
10560109 10560140 31863 31894 + cds coding_transcript
10560109 10560140 31863 31894 + exon coding_transcript
10560109 10560140 31863 31894 + cds coding_transcript
10559606 10560108 31895 32397 + intron coding_transcript
10559606 10560108 31895 32397 + intron coding_transcript
10559422 10559605 32398 32581 + exon coding_transcript
10559422 10559605 32398 32581 + cds coding_transcript
10559422 10559605 32398 32581 + exon coding_transcript
10559422 10559605 32398 32581 + cds coding_transcript
10559357 10559421 32582 32646 + intron coding_transcript
10559357 10559421 32582 32646 + intron coding_transcript
10558427 10559356 32647 34638 + exon coding_transcript
10558427 10559356 32647 34638 + exon coding_transcript
10559217 10559356 32647 33848 + cds coding_transcript
10559217 10559356 32647 33848 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 32784 32843 + cds 2xTY1 CLC
1548 2264 32850 33566 + cds SGFP CLC
2271 2312 33573 33614 + cds V5 CLC
2313 2336 33615 33638 + cds Precision cut site CLC
2337 2357 33639 33659 + cds TEV CLC
2358 2429 33660 33731 + cds BLRP CLC
3813 3846 33739 33772 + misc_recomb FRT CLC
dmel-5.43-3R
10558427 10559216 33849 34638 + three_prime_utr coding_transcript
10558427 10559216 33849 34638 + three_prime_utr coding_transcript
10550472 10557231 35834 42593 + gene CG7530 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
eff[32783]::S000169_fly_pretag_fwd
GGGAAAAATACAATGAGCTGGCACGAGAGTGGACTAGAAAGTATGCTATGgaagtgcataccaatcaggacccgc
fasta Fasta
eff[32783]::S000169_fly_pretag_rev
GATGTTGAATTTGCTGATTCTGTTGGACTGCTGCAATCTGGAACGCATCActtgtcgtcgtcatccttgtagtca
fasta Fasta