Clone: 1367792136965469 E12
Tagged gene: eff
Identifier: FBgn0011217
Clone | FlyFos023128(pRedFlp-Hgr)(eff[32783]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
---|---|
Library | FlyFos 5.43 D.mel in E. coli EPI300 |
Genome | Species: Drosophila melanogaster, Release: 5.43 |
Validation(click to hide details)
Type | Status | Download / View |
---|---|---|
Grows in selection | good | |
NGS-based tag validation | good | [view] |
Genes in pFlyFos vector(click to hide details)
Gene | Clone relative | Genome relative | |||||
---|---|---|---|---|---|---|---|
Name | Length | Start | End | Strand | Start | End | Strand |
DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
---|---|---|---|---|---|---|---|---|
pFlyFos | ||||||||
1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
dmel-5.43-3R | ||||||||
10571623 | 10573043 | 18960 | 20380 | + | gene | smp-30 | coding_transcript | |
10558427 | 10566933 | 25070 | 34638 | + | gene | eff | coding_transcript | |
10558427 | 10566933 | 25070 | 34638 | + | mrna | FBtr0083018 | coding_transcript | |
10566729 | 10566933 | 25070 | 25274 | + | exon | coding_transcript | ||
10566729 | 10566933 | 25070 | 25274 | + | five_prime_utr | coding_transcript | ||
10566663 | 10566728 | 25275 | 25340 | + | intron | coding_transcript | ||
10566102 | 10566662 | 25341 | 25901 | + | exon | coding_transcript | ||
10566126 | 10566662 | 25341 | 25877 | + | five_prime_utr | coding_transcript | ||
10558427 | 10566486 | 25517 | 34638 | + | mrna | FBtr0310554 | coding_transcript | |
10566102 | 10566486 | 25517 | 25901 | + | exon | coding_transcript | ||
10566126 | 10566486 | 25517 | 25877 | + | five_prime_utr | coding_transcript | ||
10566102 | 10566125 | 25878 | 25901 | + | cds | coding_transcript | ||
10566102 | 10566125 | 25878 | 25901 | + | cds | coding_transcript | ||
10563206 | 10566101 | 25902 | 28797 | + | intron | coding_transcript | ||
10563206 | 10566101 | 25902 | 28797 | + | intron | coding_transcript | ||
10563142 | 10563205 | 28798 | 28861 | + | exon | coding_transcript | ||
10563142 | 10563205 | 28798 | 28861 | + | cds | coding_transcript | ||
10563142 | 10563205 | 28798 | 28861 | + | exon | coding_transcript | ||
10563142 | 10563205 | 28798 | 28861 | + | cds | coding_transcript | ||
10560141 | 10563141 | 28862 | 31862 | + | intron | coding_transcript | ||
10560141 | 10563141 | 28862 | 31862 | + | intron | coding_transcript | ||
10560109 | 10560140 | 31863 | 31894 | + | exon | coding_transcript | ||
10560109 | 10560140 | 31863 | 31894 | + | cds | coding_transcript | ||
10560109 | 10560140 | 31863 | 31894 | + | exon | coding_transcript | ||
10560109 | 10560140 | 31863 | 31894 | + | cds | coding_transcript | ||
10559606 | 10560108 | 31895 | 32397 | + | intron | coding_transcript | ||
10559606 | 10560108 | 31895 | 32397 | + | intron | coding_transcript | ||
10559422 | 10559605 | 32398 | 32581 | + | exon | coding_transcript | ||
10559422 | 10559605 | 32398 | 32581 | + | cds | coding_transcript | ||
10559422 | 10559605 | 32398 | 32581 | + | exon | coding_transcript | ||
10559422 | 10559605 | 32398 | 32581 | + | cds | coding_transcript | ||
10559357 | 10559421 | 32582 | 32646 | + | intron | coding_transcript | ||
10559357 | 10559421 | 32582 | 32646 | + | intron | coding_transcript | ||
10558427 | 10559356 | 32647 | 34638 | + | exon | coding_transcript | ||
10558427 | 10559356 | 32647 | 34638 | + | exon | coding_transcript | ||
10559217 | 10559356 | 32647 | 33848 | + | cds | coding_transcript | ||
10559217 | 10559356 | 32647 | 33848 | + | cds | coding_transcript | ||
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
1482 | 1541 | 32784 | 32843 | + | cds | 2xTY1 | CLC | |
1548 | 2264 | 32850 | 33566 | + | cds | SGFP | CLC | |
2271 | 2312 | 33573 | 33614 | + | cds | V5 | CLC | |
2313 | 2336 | 33615 | 33638 | + | cds | Precision cut site | CLC | |
2337 | 2357 | 33639 | 33659 | + | cds | TEV | CLC | |
2358 | 2429 | 33660 | 33731 | + | cds | BLRP | CLC | |
3813 | 3846 | 33739 | 33772 | + | misc_recomb | FRT | CLC | |
dmel-5.43-3R | ||||||||
10558427 | 10559216 | 33849 | 34638 | + | three_prime_utr | coding_transcript | ||
10558427 | 10559216 | 33849 | 34638 | + | three_prime_utr | coding_transcript | ||
10550472 | 10557231 | 35834 | 42593 | + | gene | CG7530 | coding_transcript |
Sets(click to hide details)
Oligos(click to hide details)
Name/Sequence | Export |
---|---|
eff[32783]::S000169_fly_pretag_fwd
GGGAAAAATACAATGAGCTGGCACGAGAGTGGACTAGAAAGTATGCTATGgaagtgcataccaatcaggacccgc
|
Fasta |
eff[32783]::S000169_fly_pretag_rev
GATGTTGAATTTGCTGATTCTGTTGGACTGCTGCAATCTGGAACGCATCActtgtcgtcgtcatccttgtagtca
|
Fasta |