Clone: 1367792136965469 D12

Tagged gene: mute
Identifier: FBgn0085444

Clone FlyFos023127(pRedFlp-Hgr)(mute[38512]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
12846448 12847652 27084 28288 + gene CG9010 coding_transcript
12845007 12846436 28300 29729 - gene CG6665 coding_transcript
12842969 12844826 29910 31767 - gene veg coding_transcript
12835802 12842770 31966 39996 + gene mute coding_transcript
12835802 12842770 31966 39996 + mrna FBtr0112691 coding_transcript
12842160 12842770 31966 32576 + exon coding_transcript
12842661 12842770 31966 32075 + five_prime_utr coding_transcript
12842160 12842660 32076 32576 + cds coding_transcript
12842095 12842159 32577 32641 + intron coding_transcript
12841764 12842094 32642 32972 + exon coding_transcript
12841764 12842094 32642 32972 + cds coding_transcript
12841710 12841763 32973 33026 + intron coding_transcript
12840641 12841709 33027 34095 + exon coding_transcript
12840641 12841709 33027 34095 + cds coding_transcript
12840581 12840640 34096 34155 + intron coding_transcript
12840109 12840580 34156 34627 + exon coding_transcript
12840109 12840580 34156 34627 + cds coding_transcript
12839256 12840108 34628 35480 + intron coding_transcript
12839402 12840086 34650 35334 - gene CG34191 coding_transcript
12839402 12840086 34650 35334 - mrna FBtr0112384 coding_transcript
12839657 12840086 34650 35079 - exon coding_transcript
12840007 12840086 34650 34729 - three_prime_utr coding_transcript
12839743 12840006 34730 34993 - cds coding_transcript
12839657 12839742 34994 35079 - five_prime_utr coding_transcript
12839559 12839656 35080 35177 - intron coding_transcript
12839402 12839558 35178 35334 - exon coding_transcript
12839402 12839558 35178 35334 - five_prime_utr coding_transcript
12835802 12839318 35418 39996 + mrna FBtr0112692 coding_transcript
12838247 12839318 35418 36489 + exon coding_transcript
12838407 12839318 35418 36329 + five_prime_utr coding_transcript
12838247 12839255 35481 36489 + exon coding_transcript
12838247 12839255 35481 36489 + cds coding_transcript
12838247 12838406 36330 36489 + cds coding_transcript
12838180 12838246 36490 36556 + intron coding_transcript
12838180 12838246 36490 36556 + intron coding_transcript
12838046 12838179 36557 36690 + exon coding_transcript
12838046 12838179 36557 36690 + cds coding_transcript
12838046 12838179 36557 36690 + exon coding_transcript
12838046 12838179 36557 36690 + cds coding_transcript
12837986 12838045 36691 36750 + intron coding_transcript
12837986 12838045 36691 36750 + intron coding_transcript
12837657 12837985 36751 37079 + exon coding_transcript
12837657 12837985 36751 37079 + cds coding_transcript
12837657 12837985 36751 37079 + exon coding_transcript
12837657 12837985 36751 37079 + cds coding_transcript
12837596 12837656 37080 37140 + intron coding_transcript
12837596 12837656 37080 37140 + intron coding_transcript
12835802 12837595 37141 39996 + exon coding_transcript
12835802 12837595 37141 39996 + exon coding_transcript
12836221 12837595 37141 39577 + cds coding_transcript
12836221 12837595 37141 39577 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 38513 38572 + cds 2xTY1 CLC
1548 2264 38579 39295 + cds SGFP CLC
2271 2312 39302 39343 + cds V5 CLC
2313 2336 39344 39367 + cds Precision cut site CLC
2337 2357 39368 39388 + cds TEV CLC
2358 2429 39389 39460 + cds BLRP CLC
3813 3846 39468 39501 + misc_recomb FRT CLC
dmel-5.43-2R
12835802 12836220 39578 39996 + three_prime_utr coding_transcript
12835802 12836220 39578 39996 + three_prime_utr coding_transcript
12835163 12835729 40069 40635 + gene Vha16-4 coding_transcript
12834710 12835018 40780 41088 + gene CG34190 coding_transcript
12833060 12834736 41062 42738 - gene CG15614 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
mute[38512]::S000169_fly_pretag_fwd
GTAGTAGGCATCAGTTTCTTATGGACTTTCTGTCCAAATTGCAGGGAAAGgaagtgcataccaatcaggacccgc
fasta Fasta
mute[38512]::S000169_fly_pretag_rev
TATTTCAGCTTGGATTATAATACACAAAAGACCATCTTAGAGAAACCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta