Clone: 1367792136965469 F08

Tagged gene: Sfp23F
Identifier: FBgn0259949

Clone FlyFos023095(pRedFlp-Hgr)(Sfp23F[16658]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
3387898 3388496 15569 16167 + gene CG42460 coding_transcript
3387392 3387743 16322 17735 + gene Sfp23F coding_transcript
3387392 3387743 16322 17735 + mrna FBtr0300295 coding_transcript
3387642 3387743 16322 16423 + exon coding_transcript
3387709 3387743 16322 16356 + five_prime_utr coding_transcript
3387642 3387708 16357 16423 + cds coding_transcript
3387574 3387641 16424 16491 + intron coding_transcript
3387392 3387573 16492 17735 + exon coding_transcript
3387404 3387573 16492 17723 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 16659 16718 + cds 2xTY1 CLC
1548 2264 16725 17441 + cds SGFP CLC
2271 2312 17448 17489 + cds V5 CLC
2313 2336 17490 17513 + cds Precision cut site CLC
2337 2357 17514 17534 + cds TEV CLC
2358 2429 17535 17606 + cds BLRP CLC
3813 3846 17614 17647 + misc_recomb FRT CLC
dmel-5.43-2L
3387392 3387403 17724 17735 + three_prime_utr coding_transcript
3377008 3378586 26541 28119 - gene Ptpa coding_transcript
3375883 3376686 28441 29244 + gene CG9662 coding_transcript
3373567 3375711 29416 31560 + gene CG15412 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Sfp23F[16658]::S000169_fly_pretag_fwd
ATGGAACTTCATTTGAGAGCATTAAGGAATGCGAAGATAAATGCAAAGAGgaagtgcataccaatcaggacccgc
fasta Fasta
Sfp23F[16658]::S000169_fly_pretag_rev
ACTTTTTGAAATATTTAATATTGTTTTTTTATTTTACAGCCATAAGACTActtgtcgtcgtcatccttgtagtca
fasta Fasta