Clone: 1367792136965469 A02

Tagged gene: decay
Identifier: FBgn0028381

Clone FlyFos022999(pRedFlp-Hgr)(decay[22813]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
12323539 12324681 21433 22575 - gene Gr89a coding_transcript
12324780 12327394 22674 26350 - gene decay coding_transcript
12324780 12327394 22674 26350 - mrna FBtr0083304 coding_transcript
12324780 12325179 22674 24135 - exon coding_transcript
12324780 12324916 22674 22810 - three_prime_utr coding_transcript
12324917 12325179 22811 24135 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 22887 22920 - misc_recomb FRT CLC
2358 2429 22928 22999 - cds BLRP CLC
2337 2357 23000 23020 - cds TEV CLC
2313 2336 23021 23044 - cds Precision cut site CLC
2271 2312 23045 23086 - cds V5 CLC
1548 2264 23093 23809 - cds SGFP CLC
1482 1541 23816 23875 - cds 2xTY1 CLC
dmel-5.43-3R
12325180 12325484 24136 24440 - intron coding_transcript
12325485 12325817 24441 24773 - exon coding_transcript
12325485 12325817 24441 24773 - cds coding_transcript
12325818 12326394 24774 25350 - intron coding_transcript
12326395 12327394 25351 26350 - exon coding_transcript
12326395 12326725 25351 25681 - cds coding_transcript
12326726 12327394 25682 26350 - five_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
decay[22813]::S000169_fly_pretag_rev
CTGACTCACAGTCCCGATTGGATTGTCGCTGATTTCGTGACCGGAGCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta
decay[22813]::S000169_fly_pretag_fwd
TTATGTCGACACTGACCAAAACATTCCAGCTGCGTGTTAAGCCCAAGACCgaagtgcataccaatcaggacccgc
fasta Fasta