Clone: 7882854381648677 D12

Tagged gene: mRpL53
Identifier: FBgn0050481

Clone FlyFos021695(pRedFlp-Hgr)(mRpL53[30077]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
9830039 9830882 17206 18049 - gene DJ-1alpha coding_transcript
9830892 9845594 18059 33823 - gene AGO1 coding_transcript
9841704 9843174 28871 31403 + gene CG33155 coding_transcript
9841704 9843174 28871 31403 + mrna FBtr0100452 coding_transcript
9841704 9843174 28871 31403 + gene mRpL53 coding_transcript
9841704 9843174 28871 31403 + mrna FBtr0100285 coding_transcript
9841704 9841950 28871 29117 + exon coding_transcript
9841704 9841950 28871 29117 + five_prime_utr coding_transcript
9841704 9841950 28871 29117 + exon coding_transcript
9841704 9841804 28871 28971 + five_prime_utr coding_transcript
9841805 9841950 28972 29117 + cds coding_transcript
9841951 9842421 29118 29588 + intron coding_transcript
9841951 9842421 29118 29588 + intron coding_transcript
9842003 9843174 29170 31403 + mrna FBtr0100451 coding_transcript
9842003 9842149 29170 29316 + exon coding_transcript
9842003 9842149 29170 29316 + five_prime_utr coding_transcript
9842032 9843174 29199 31403 + mrna FBtr0100453 coding_transcript
9842032 9842366 29199 29533 + exon coding_transcript
9842032 9842366 29199 29533 + five_prime_utr coding_transcript
9842150 9842421 29317 29588 + intron coding_transcript
9842192 9842354 29359 29521 - exon coding_transcript
9842192 9842354 29359 29521 - exon coding_transcript
9842192 9842354 29359 29521 - exon coding_transcript
9842192 9842312 29359 29479 - cds coding_transcript
9842192 9842312 29359 29479 - cds coding_transcript
9842192 9842312 29359 29479 - cds coding_transcript
9842313 9842354 29480 29521 - five_prime_utr coding_transcript
9842313 9842354 29480 29521 - five_prime_utr coding_transcript
9842313 9842354 29480 29521 - five_prime_utr coding_transcript
9842355 9843136 29522 31365 - intron coding_transcript
9842367 9842421 29534 29588 + intron coding_transcript
9842422 9842549 29589 29716 + exon coding_transcript
9842422 9842549 29589 29716 + five_prime_utr coding_transcript
9842422 9842549 29589 29716 + exon coding_transcript
9842422 9842549 29589 29716 + five_prime_utr coding_transcript
9842422 9842549 29589 29716 + exon coding_transcript
9842422 9842549 29589 29716 + five_prime_utr coding_transcript
9842422 9842549 29589 29716 + exon coding_transcript
9842422 9842549 29589 29716 + cds coding_transcript
9842550 9842719 29717 29886 + intron coding_transcript
9842550 9842719 29717 29886 + intron coding_transcript
9842550 9842719 29717 29886 + intron coding_transcript
9842550 9842719 29717 29886 + intron coding_transcript
9842720 9843174 29887 31403 + exon coding_transcript
9842720 9843174 29887 31403 + exon coding_transcript
9842720 9843174 29887 31403 + exon coding_transcript
9842720 9843174 29887 31403 + exon coding_transcript
9842720 9842921 29887 31150 + five_prime_utr coding_transcript
9842720 9842921 29887 31150 + five_prime_utr coding_transcript
9842720 9842921 29887 31150 + five_prime_utr coding_transcript
9842720 9842913 29887 31142 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 30078 30137 + cds 2xTY1 CLC
1548 2264 30144 30860 + cds SGFP CLC
2271 2312 30867 30908 + cds V5 CLC
2313 2336 30909 30932 + cds Precision cut site CLC
2337 2357 30933 30953 + cds TEV CLC
2358 2429 30954 31025 + cds BLRP CLC
3813 3846 31033 31066 + misc_recomb FRT CLC
dmel-5.43-2R
9842914 9843174 31143 31403 + three_prime_utr coding_transcript
9842922 9843104 31151 31333 + cds coding_transcript
9842922 9843104 31151 31333 + cds coding_transcript
9842922 9843104 31151 31333 + cds coding_transcript
9843105 9843174 31334 31403 + three_prime_utr coding_transcript
9843105 9843174 31334 31403 + three_prime_utr coding_transcript
9843105 9843174 31334 31403 + three_prime_utr coding_transcript
9846033 9847909 34262 36138 - gene CG13349 coding_transcript
9852173 9852879 40402 41108 + gene CG42806 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
mRpL53[30077]::S000169_fly_pretag_rev
CTCGATCACCGCCAGTGTTCCTTTGTGCTTCCTCACCATCCTGATGGTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
mRpL53[30077]::S000169_fly_pretag_fwd
AGAAACTAGCAGGAGGCGCCGGCGGCGGAAAGAAGGCAACCAAGAAGAAGgaagtgcataccaatcaggacccgc
 fasta Fasta