Clone: 7882854381648677 F10
Tagged gene: RpS25
Identifier: FBgn0086472
| Clone | FlyFos021676(pRedFlp-Hgr)(RpS25[30414]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation(click to hide details)
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | good | [view] |
Genes in pFlyFos vector(click to hide details)
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-3R | ||||||||
| 7021826 | 7023007 | 10965 | 12146 | + | gene | CG18577 | coding_transcript | |
| 7026138 | 7034357 | 15277 | 23496 | + | gene | cu | coding_transcript | |
| 7034856 | 7035571 | 23995 | 24710 | - | gene | SdhC | coding_transcript | |
| 7035975 | 7037004 | 25114 | 26143 | + | gene | Tctp | coding_transcript | |
| 7038030 | 7040239 | 27169 | 29378 | - | gene | CG6672 | coding_transcript | |
| 7040750 | 7042005 | 29889 | 32206 | - | gene | RpS25 | coding_transcript | |
| 7040750 | 7042005 | 29889 | 32206 | - | mrna | FBtr0082369 | coding_transcript | |
| 7040750 | 7041091 | 29889 | 30230 | - | exon | coding_transcript | ||
| 7040750 | 7041091 | 29889 | 30230 | - | three_prime_utr | coding_transcript | ||
| 7041092 | 7041144 | 30231 | 30283 | - | intron | coding_transcript | ||
| 7041138 | 7042005 | 30277 | 32206 | - | mrna | FBtr0082370 | coding_transcript | |
| 7041138 | 7041346 | 30277 | 31547 | - | exon | coding_transcript | ||
| 7041138 | 7041272 | 30277 | 30411 | - | three_prime_utr | coding_transcript | ||
| 7041145 | 7041346 | 30284 | 31547 | - | exon | coding_transcript | ||
| 7041145 | 7041272 | 30284 | 30411 | - | three_prime_utr | coding_transcript | ||
| 7041273 | 7041346 | 30412 | 31547 | - | cds | coding_transcript | ||
| 7041273 | 7041346 | 30412 | 31547 | - | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 2437 | 2470 | 30488 | 30521 | - | misc_recomb | FRT | CLC | |
| 2358 | 2429 | 30529 | 30600 | - | cds | BLRP | CLC | |
| 2337 | 2357 | 30601 | 30621 | - | cds | TEV | CLC | |
| 2313 | 2336 | 30622 | 30645 | - | cds | Precision cut site | CLC | |
| 2271 | 2312 | 30646 | 30687 | - | cds | V5 | CLC | |
| 1548 | 2264 | 30694 | 31410 | - | cds | SGFP | CLC | |
| 1482 | 1541 | 31417 | 31476 | - | cds | 2xTY1 | CLC | |
| dmel-5.43-3R | ||||||||
| 7041347 | 7041414 | 31548 | 31615 | - | intron | coding_transcript | ||
| 7041347 | 7041414 | 31548 | 31615 | - | intron | coding_transcript | ||
| 7041415 | 7041691 | 31616 | 31892 | - | exon | coding_transcript | ||
| 7041415 | 7041691 | 31616 | 31892 | - | cds | coding_transcript | ||
| 7041415 | 7041691 | 31616 | 31892 | - | exon | coding_transcript | ||
| 7041415 | 7041691 | 31616 | 31892 | - | cds | coding_transcript | ||
| 7041692 | 7041980 | 31893 | 32181 | - | intron | coding_transcript | ||
| 7041692 | 7041980 | 31893 | 32181 | - | intron | coding_transcript | ||
| 7041981 | 7042005 | 32182 | 32206 | - | exon | coding_transcript | ||
| 7041981 | 7042005 | 32182 | 32206 | - | exon | coding_transcript | ||
| 7041981 | 7041983 | 32182 | 32184 | - | cds | coding_transcript | ||
| 7041981 | 7041983 | 32182 | 32184 | - | cds | coding_transcript | ||
| 7041984 | 7042005 | 32185 | 32206 | - | five_prime_utr | coding_transcript | ||
| 7041984 | 7042005 | 32185 | 32206 | - | five_prime_utr | coding_transcript | ||
| 7042333 | 7043620 | 32534 | 33821 | + | gene | CG4820 | coding_transcript | |
| 7043620 | 7045766 | 33821 | 35967 | - | gene | CG6689 | coding_transcript | |
| 7045990 | 7047361 | 36191 | 37562 | - | gene | CG6693 | coding_transcript | |
| 7047616 | 7050666 | 37817 | 40867 | + | gene | RpL3 | coding_transcript | |
| 7051242 | 7052204 | 41443 | 42405 | + | gene | scpr-C | coding_transcript | |
| 7052486 | 7053949 | 42687 | 44150 | + | gene | CG17726 | coding_transcript | |
| 7053880 | 7056291 | 44081 | 46492 | - | gene | CG10703 | coding_transcript | |
| 7056339 | 7057578 | 46540 | 47779 | - | gene | CG17187 | coding_transcript | |
| 7057634 | 7058261 | 47835 | 48462 | + | gene | CG14701 | coding_transcript | |
| 7058151 | 7060907 | 48352 | 51108 | - | gene | CG17184 | coding_transcript | |
| 7060862 | 7061799 | 51063 | 52000 | + | gene | CG17721 | coding_transcript | |
| 7062008 | 7063346 | 52209 | 53547 | + | gene | CG31441 | coding_transcript | |
Sets(click to hide details)
Oligos(click to hide details)
| Name/Sequence | Export |
|---|---|
|
RpS25[30414]::S000169_fly_pretag_rev
GAGTGCGACTACGGCGATGCGAGATGGGCAAAAGGTAAAACATTCATTTActtgtcgtcgtcatccttgtagtca
|
Fasta
|
|
RpS25[30414]::S000169_fly_pretag_fwd
AGCATCATTCCCAGGTCATCTACACACGTGCCACCAAGGGTGATGAGGCGgaagtgcataccaatcaggacccgc
|
Fasta
|