Clone: 1213189838248655 G01

Tagged gene: Spt20
Identifier: FBgn0036374

Clone FlyFos019648(pRedFlp-Hgr)(Spt20[30633]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
13495840 13503795 23578 32595 + gene Spt20 coding_transcript
13495840 13503795 23578 32595 + mrna FBtr0075812 coding_transcript
13495840 13503795 23578 32595 + mrna FBtr0304904 coding_transcript
13495840 13496484 23578 24222 + exon coding_transcript
13495840 13496484 23578 24222 + exon coding_transcript
13495840 13496448 23578 24186 + five_prime_utr coding_transcript
13495840 13496448 23578 24186 + five_prime_utr coding_transcript
13496449 13496484 24187 24222 + cds coding_transcript
13496449 13496484 24187 24222 + cds coding_transcript
13496485 13496555 24223 24293 + intron coding_transcript
13496485 13496555 24223 24293 + intron coding_transcript
13496556 13497497 24294 25235 + exon coding_transcript
13496556 13497497 24294 25235 + cds coding_transcript
13496556 13497497 24294 25235 + exon coding_transcript
13496556 13497497 24294 25235 + cds coding_transcript
13497498 13497555 25236 25293 + intron coding_transcript
13497498 13497555 25236 25293 + intron coding_transcript
13497556 13499670 25294 27408 + exon coding_transcript
13497556 13499670 25294 27408 + cds coding_transcript
13497556 13499670 25294 27408 + exon coding_transcript
13497556 13499670 25294 27408 + cds coding_transcript
13499671 13499739 27409 27477 + intron coding_transcript
13499671 13499739 27409 27477 + intron coding_transcript
13499740 13499971 27478 27709 + exon coding_transcript
13499740 13499971 27478 27709 + cds coding_transcript
13499740 13499971 27478 27709 + exon coding_transcript
13499740 13499971 27478 27709 + cds coding_transcript
13499972 13500034 27710 27772 + intron coding_transcript
13499972 13500034 27710 27772 + intron coding_transcript
13500035 13501645 27773 29383 + exon coding_transcript
13500035 13501645 27773 29383 + cds coding_transcript
13500035 13501645 27773 29383 + exon coding_transcript
13500035 13501645 27773 29383 + cds coding_transcript
13501646 13502149 29384 29887 + intron coding_transcript
13501646 13501975 29384 29713 + intron coding_transcript
13501976 13502434 29714 30172 + exon coding_transcript
13501976 13501980 29714 29718 + cds coding_transcript
13501981 13502434 29719 30172 + three_prime_utr coding_transcript
13502150 13502434 29888 30172 + exon coding_transcript
13502150 13502434 29888 30172 + cds coding_transcript
13502435 13502497 30173 30235 + intron coding_transcript
13502435 13502497 30173 30235 + intron coding_transcript
13502498 13503795 30236 32595 + exon coding_transcript
13502498 13503795 30236 32595 + exon coding_transcript
13502498 13503795 30236 32595 + three_prime_utr coding_transcript
13502498 13502898 30236 31698 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 30634 30693 + cds 2xTY1 CLC
1548 2264 30700 31416 + cds SGFP CLC
2271 2312 31423 31464 + cds V5 CLC
2313 2336 31465 31488 + cds Precision cut site CLC
2337 2357 31489 31509 + cds TEV CLC
2358 2429 31510 31581 + cds BLRP CLC
3813 3846 31589 31622 + misc_recomb FRT CLC
dmel-5.43-3L
13502899 13503795 31699 32595 + three_prime_utr coding_transcript
13504746 13506207 33546 35007 - gene Vps36 coding_transcript
13506354 13509376 35154 38176 + gene Liprin-beta coding_transcript
13509373 13512701 38173 41501 - gene CG10710 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Spt20[30633]::S000169_fly_pretag_rev
TCTCAGTCGTCCCAATAGTCGTCTCAGTTCCAGGCAATTCACATCCCTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Spt20[30633]::S000169_fly_pretag_fwd
AACAACAGGCTGCGGTCAATCGACGGCGCTCCACCACCGATGCGACCAAGgaagtgcataccaatcaggacccgc
fasta Fasta