Clone: 1213189838248655 G01
Tagged gene: Spt20
Identifier: FBgn0036374
Clone | FlyFos019648(pRedFlp-Hgr)(Spt20[30633]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
---|---|
Library | FlyFos 5.43 D.mel in E. coli EPI300 |
Genome | Species: Drosophila melanogaster, Release: 5.43 |
Validation(click to hide details)
Type | Status | Download / View |
---|---|---|
Grows in selection | good | |
NGS-based tag validation | good | [view] |
Genes in pFlyFos vector(click to hide details)
Gene | Clone relative | Genome relative | |||||
---|---|---|---|---|---|---|---|
Name | Length | Start | End | Strand | Start | End | Strand |
DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
---|---|---|---|---|---|---|---|---|
pFlyFos | ||||||||
1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
dmel-5.43-3L | ||||||||
13495840 | 13503795 | 23578 | 32595 | + | gene | Spt20 | coding_transcript | |
13495840 | 13503795 | 23578 | 32595 | + | mrna | FBtr0075812 | coding_transcript | |
13495840 | 13503795 | 23578 | 32595 | + | mrna | FBtr0304904 | coding_transcript | |
13495840 | 13496484 | 23578 | 24222 | + | exon | coding_transcript | ||
13495840 | 13496484 | 23578 | 24222 | + | exon | coding_transcript | ||
13495840 | 13496448 | 23578 | 24186 | + | five_prime_utr | coding_transcript | ||
13495840 | 13496448 | 23578 | 24186 | + | five_prime_utr | coding_transcript | ||
13496449 | 13496484 | 24187 | 24222 | + | cds | coding_transcript | ||
13496449 | 13496484 | 24187 | 24222 | + | cds | coding_transcript | ||
13496485 | 13496555 | 24223 | 24293 | + | intron | coding_transcript | ||
13496485 | 13496555 | 24223 | 24293 | + | intron | coding_transcript | ||
13496556 | 13497497 | 24294 | 25235 | + | exon | coding_transcript | ||
13496556 | 13497497 | 24294 | 25235 | + | cds | coding_transcript | ||
13496556 | 13497497 | 24294 | 25235 | + | exon | coding_transcript | ||
13496556 | 13497497 | 24294 | 25235 | + | cds | coding_transcript | ||
13497498 | 13497555 | 25236 | 25293 | + | intron | coding_transcript | ||
13497498 | 13497555 | 25236 | 25293 | + | intron | coding_transcript | ||
13497556 | 13499670 | 25294 | 27408 | + | exon | coding_transcript | ||
13497556 | 13499670 | 25294 | 27408 | + | cds | coding_transcript | ||
13497556 | 13499670 | 25294 | 27408 | + | exon | coding_transcript | ||
13497556 | 13499670 | 25294 | 27408 | + | cds | coding_transcript | ||
13499671 | 13499739 | 27409 | 27477 | + | intron | coding_transcript | ||
13499671 | 13499739 | 27409 | 27477 | + | intron | coding_transcript | ||
13499740 | 13499971 | 27478 | 27709 | + | exon | coding_transcript | ||
13499740 | 13499971 | 27478 | 27709 | + | cds | coding_transcript | ||
13499740 | 13499971 | 27478 | 27709 | + | exon | coding_transcript | ||
13499740 | 13499971 | 27478 | 27709 | + | cds | coding_transcript | ||
13499972 | 13500034 | 27710 | 27772 | + | intron | coding_transcript | ||
13499972 | 13500034 | 27710 | 27772 | + | intron | coding_transcript | ||
13500035 | 13501645 | 27773 | 29383 | + | exon | coding_transcript | ||
13500035 | 13501645 | 27773 | 29383 | + | cds | coding_transcript | ||
13500035 | 13501645 | 27773 | 29383 | + | exon | coding_transcript | ||
13500035 | 13501645 | 27773 | 29383 | + | cds | coding_transcript | ||
13501646 | 13502149 | 29384 | 29887 | + | intron | coding_transcript | ||
13501646 | 13501975 | 29384 | 29713 | + | intron | coding_transcript | ||
13501976 | 13502434 | 29714 | 30172 | + | exon | coding_transcript | ||
13501976 | 13501980 | 29714 | 29718 | + | cds | coding_transcript | ||
13501981 | 13502434 | 29719 | 30172 | + | three_prime_utr | coding_transcript | ||
13502150 | 13502434 | 29888 | 30172 | + | exon | coding_transcript | ||
13502150 | 13502434 | 29888 | 30172 | + | cds | coding_transcript | ||
13502435 | 13502497 | 30173 | 30235 | + | intron | coding_transcript | ||
13502435 | 13502497 | 30173 | 30235 | + | intron | coding_transcript | ||
13502498 | 13503795 | 30236 | 32595 | + | exon | coding_transcript | ||
13502498 | 13503795 | 30236 | 32595 | + | exon | coding_transcript | ||
13502498 | 13503795 | 30236 | 32595 | + | three_prime_utr | coding_transcript | ||
13502498 | 13502898 | 30236 | 31698 | + | cds | coding_transcript | ||
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
1482 | 1541 | 30634 | 30693 | + | cds | 2xTY1 | CLC | |
1548 | 2264 | 30700 | 31416 | + | cds | SGFP | CLC | |
2271 | 2312 | 31423 | 31464 | + | cds | V5 | CLC | |
2313 | 2336 | 31465 | 31488 | + | cds | Precision cut site | CLC | |
2337 | 2357 | 31489 | 31509 | + | cds | TEV | CLC | |
2358 | 2429 | 31510 | 31581 | + | cds | BLRP | CLC | |
3813 | 3846 | 31589 | 31622 | + | misc_recomb | FRT | CLC | |
dmel-5.43-3L | ||||||||
13502899 | 13503795 | 31699 | 32595 | + | three_prime_utr | coding_transcript | ||
13504746 | 13506207 | 33546 | 35007 | - | gene | Vps36 | coding_transcript | |
13506354 | 13509376 | 35154 | 38176 | + | gene | Liprin-beta | coding_transcript | |
13509373 | 13512701 | 38173 | 41501 | - | gene | CG10710 | coding_transcript |
Sets(click to hide details)
Oligos(click to hide details)
Name/Sequence | Export |
---|---|
Spt20[30633]::S000169_fly_pretag_rev
TCTCAGTCGTCCCAATAGTCGTCTCAGTTCCAGGCAATTCACATCCCTTActtgtcgtcgtcatccttgtagtca
|
Fasta |
Spt20[30633]::S000169_fly_pretag_fwd
AACAACAGGCTGCGGTCAATCGACGGCGCTCCACCACCGATGCGACCAAGgaagtgcataccaatcaggacccgc
|
Fasta |