Clone: 1213189838248655 E01

Tagged gene: Gycalpha99B
Identifier: FBgn0013972

Clone FlyFos019644(pRedFlp-Hgr)(Gycalpha99B[19485]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
25511458 25512501 18318 19361 + gene Obp99c coding_transcript
25508225 25511480 19339 23656 - gene Gycalpha99B coding_transcript
25508225 25511480 19339 23656 - mrna FBtr0085453 coding_transcript
25511132 25511480 19339 20749 - exon coding_transcript
25511337 25511480 19339 19482 - three_prime_utr coding_transcript
25511132 25511336 19483 20749 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19559 19592 - misc_recomb FRT CLC
2358 2429 19600 19671 - cds BLRP CLC
2337 2357 19672 19692 - cds TEV CLC
2313 2336 19693 19716 - cds Precision cut site CLC
2271 2312 19717 19758 - cds V5 CLC
1548 2264 19765 20481 - cds SGFP CLC
1482 1541 20488 20547 - cds 2xTY1 CLC
dmel-5.43-3R
25511070 25511131 20750 20811 - intron coding_transcript
25509721 25511069 20812 22160 - exon coding_transcript
25509721 25511069 20812 22160 - cds coding_transcript
25509661 25509720 22161 22220 - intron coding_transcript
25509420 25509660 22221 22461 - exon coding_transcript
25509420 25509660 22221 22461 - cds coding_transcript
25509360 25509419 22462 22521 - intron coding_transcript
25509088 25509359 22522 22793 - exon coding_transcript
25509124 25509359 22522 22757 - cds coding_transcript
25509088 25509123 22758 22793 - five_prime_utr coding_transcript
25508368 25509087 22794 23513 - intron coding_transcript
25508225 25508367 23514 23656 - exon coding_transcript
25508225 25508367 23514 23656 - five_prime_utr coding_transcript
25505963 25508000 23881 25918 + gene CG7582 coding_transcript
25501399 25505979 25902 30482 - gene CAP-D2 coding_transcript
25499855 25501141 30740 32026 + gene Bub3 coding_transcript
25498178 25499945 31936 33703 - gene ppk19 coding_transcript
25497382 25498056 33825 34499 - gene Obp99a coding_transcript
25494871 25496972 34909 37010 - gene CG18110 coding_transcript
25492536 25494599 37282 39345 + gene ppk20 coding_transcript
25490501 25492505 39376 41380 - gene ppk21 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Gycalpha99B[19485]::S000169_fly_pretag_rev
TACTGAGCGTGTGGGTGGTCTACGGGGGGATTGGAGCTACGGGGGGTCTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Gycalpha99B[19485]::S000169_fly_pretag_fwd
TGGAGCACATCAATGTGTCCATGAAGACCATATCCGAGGGCGGCGATGCCgaagtgcataccaatcaggacccgc
fasta Fasta