Clone: 1213189838248655 D01

Tagged gene: Bub3
Identifier: FBgn0025457

Clone FlyFos019644(pRedFlp-Hgr)(Bub3[30819]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
25511458 25512501 18318 19361 + gene Obp99c coding_transcript
25508225 25511480 19339 22594 - gene Gycalpha99B coding_transcript
25505963 25508000 22819 24856 + gene CG7582 coding_transcript
25501399 25505979 24840 29420 - gene CAP-D2 coding_transcript
25499855 25501141 29678 32026 + gene Bub3 coding_transcript
25499855 25501141 29678 32026 + mrna FBtr0085465 coding_transcript
25500839 25501141 29678 29980 + exon coding_transcript
25501031 25501141 29678 29788 + five_prime_utr coding_transcript
25500839 25501030 29789 29980 + cds coding_transcript
25500786 25500838 29981 30033 + intron coding_transcript
25499855 25500785 30034 32026 + exon coding_transcript
25499997 25500785 30034 31884 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 30820 30879 + cds 2xTY1 CLC
1548 2264 30886 31602 + cds SGFP CLC
2271 2312 31609 31650 + cds V5 CLC
2313 2336 31651 31674 + cds Precision cut site CLC
2337 2357 31675 31695 + cds TEV CLC
2358 2429 31696 31767 + cds BLRP CLC
3813 3846 31775 31808 + misc_recomb FRT CLC
dmel-5.43-3R
25499855 25499996 31885 32026 + three_prime_utr coding_transcript
25498178 25499945 31936 33703 - gene ppk19 coding_transcript
25497382 25498056 33825 34499 - gene Obp99a coding_transcript
25494871 25496972 34909 37010 - gene CG18110 coding_transcript
25492536 25494599 37282 39345 + gene ppk20 coding_transcript
25490501 25492505 39376 41380 - gene ppk21 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Bub3[30819]::S000169_fly_pretag_rev
CTTTAAAAAATGATGTAAAACTTTGCCGTAAACAAGTATATATAACATTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
Bub3[30819]::S000169_fly_pretag_fwd
ATCCGGCTATCTATATACGCTATCCCACGGACCAGGAAACTAAACAGAAAgaagtgcataccaatcaggacccgc
 fasta Fasta