Clone: 0864533050210965 G12

Tagged gene: Acp53C14c
Identifier: FBgn0053530

Clone FlyFos018977(pRedFlp-Hgr)(Acp53C14c[31504]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
12639008 12660002 9813 30807 + gene unc-104 coding_transcript
12654342 12656743 13072 15473 - gene CG5348 coding_transcript
12652866 12653433 16382 16949 + gene CG42392 coding_transcript
12642606 12643328 26487 27209 - gene CG5267 coding_transcript
12642012 12642380 27435 27803 - gene CG34189 coding_transcript
12638281 12638974 30841 32596 + gene Acp53C14c coding_transcript
12638281 12638974 30841 32596 + mrna FBtr0091496 coding_transcript
12638281 12638974 30841 32596 + mrna FBtr0303024 coding_transcript
12638955 12638974 30841 30860 + exon coding_transcript
12638955 12638974 30841 30860 + five_prime_utr coding_transcript
12638955 12638974 30841 30860 + exon coding_transcript
12638955 12638974 30841 30860 + five_prime_utr coding_transcript
12638803 12638954 30861 31012 + intron coding_transcript
12638856 12638954 30861 30959 + intron coding_transcript
12638698 12638855 30960 31117 + exon coding_transcript
12638740 12638855 30960 31075 + five_prime_utr coding_transcript
12638698 12638802 31013 31117 + exon coding_transcript
12638740 12638802 31013 31075 + five_prime_utr coding_transcript
12638698 12638739 31076 31117 + cds coding_transcript
12638698 12638739 31076 31117 + cds coding_transcript
12638641 12638697 31118 31174 + intron coding_transcript
12638641 12638697 31118 31174 + intron coding_transcript
12638281 12638640 31175 32596 + exon coding_transcript
12638281 12638640 31175 32596 + exon coding_transcript
12638308 12638640 31175 32569 + cds coding_transcript
12638308 12638640 31175 32569 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 31505 31564 + cds 2xTY1 CLC
1548 2264 31571 32287 + cds SGFP CLC
2271 2312 32294 32335 + cds V5 CLC
2313 2336 32336 32359 + cds Precision cut site CLC
2337 2357 32360 32380 + cds TEV CLC
2358 2429 32381 32452 + cds BLRP CLC
3813 3846 32460 32493 + misc_recomb FRT CLC
dmel-5.43-2R
12638281 12638307 32570 32596 + three_prime_utr coding_transcript
12638281 12638307 32570 32596 + three_prime_utr coding_transcript
12637308 12637827 33050 33569 + gene Acp53Ea coding_transcript
12636411 12636952 33925 34466 + gene Acp53C14b coding_transcript
12635565 12636077 34800 35312 + gene Acp53C14a coding_transcript
12632728 12634067 36810 38149 + gene CG15617 coding_transcript
12632055 12632609 38268 38822 + gene Sfp53D coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Acp53C14c[31504]::S000169_fly_pretag_fwd
CGCCACACCTTGACAAACTTATTAGTGGCAAGTGCCTACCAGGGCTCAGCgaagtgcataccaatcaggacccgc
fasta Fasta
Acp53C14c[31504]::S000169_fly_pretag_rev
TAAGAATTTAAAAACCAAATAAAGTGTTTGCATATGTATATATTGCTTTActtgtcgtcgtcatccttgtagtca
fasta Fasta