Clone: 0864533050210965 F12

Tagged gene: Acp53Ea
Identifier: FBgn0015584

Clone FlyFos018977(pRedFlp-Hgr)(Acp53Ea[32451]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
12639008 12660002 9813 30807 + gene unc-104 coding_transcript
12654342 12656743 13072 15473 - gene CG5348 coding_transcript
12652866 12653433 16382 16949 + gene CG42392 coding_transcript
12642606 12643328 26487 27209 - gene CG5267 coding_transcript
12642012 12642380 27435 27803 - gene CG34189 coding_transcript
12638281 12638974 30841 31534 + gene Acp53C14c coding_transcript
12637308 12637827 31988 33569 + gene Acp53Ea coding_transcript
12637308 12637827 31988 33569 + mrna FBtr0087080 coding_transcript
12637747 12637827 31988 32068 + exon coding_transcript
12637789 12637827 31988 32026 + five_prime_utr coding_transcript
12637747 12637788 32027 32068 + cds coding_transcript
12637682 12637746 32069 32133 + intron coding_transcript
12637308 12637681 32134 33569 + exon coding_transcript
12637361 12637681 32134 33516 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 32452 32511 + cds 2xTY1 CLC
1548 2264 32518 33234 + cds SGFP CLC
2271 2312 33241 33282 + cds V5 CLC
2313 2336 33283 33306 + cds Precision cut site CLC
2337 2357 33307 33327 + cds TEV CLC
2358 2429 33328 33399 + cds BLRP CLC
3813 3846 33407 33440 + misc_recomb FRT CLC
dmel-5.43-2R
12637308 12637360 33517 33569 + three_prime_utr coding_transcript
12636411 12636952 33925 34466 + gene Acp53C14b coding_transcript
12635565 12636077 34800 35312 + gene Acp53C14a coding_transcript
12632728 12634067 36810 38149 + gene CG15617 coding_transcript
12632055 12632609 38268 38822 + gene Sfp53D coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Acp53Ea[32451]::S000169_fly_pretag_fwd
TACTGAGGCCTTATGTAAAATCGCTTGAGACCAACAAATGCTTGGGTGAAgaagtgcataccaatcaggacccgc
fasta Fasta
Acp53Ea[32451]::S000169_fly_pretag_rev
CTTTATTAATGCTCAGAAATTTATTTTATTATGGCCAAAATAGTGATTTActtgtcgtcgtcatccttgtagtca
fasta Fasta