Clone: 0864533050210965 B12

Tagged gene: Sb
Identifier: FBgn0003319

Clone FlyFos018962(pRedFlp-Hgr)(Sb[37473]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
11954451 11971006 21443 39060 + gene Sb coding_transcript
11954451 11971006 21443 39060 + mrna FBtr0083250 coding_transcript
11954451 11954602 21443 21594 + exon coding_transcript
11954451 11954602 21443 21594 + five_prime_utr coding_transcript
11954603 11957948 21595 24940 + intron coding_transcript
11957949 11958666 24941 25658 + exon coding_transcript
11957949 11958666 24941 25658 + five_prime_utr coding_transcript
11958667 11965546 25659 32538 + intron coding_transcript
11965547 11965609 32539 32601 + exon coding_transcript
11965547 11965568 32539 32560 + five_prime_utr coding_transcript
11965569 11965609 32561 32601 + cds coding_transcript
11965610 11966304 32602 33296 + intron coding_transcript
11966305 11966904 33297 33896 + exon coding_transcript
11966305 11966904 33297 33896 + cds coding_transcript
11966905 11967100 33897 34092 + intron coding_transcript
11967101 11967294 34093 34286 + exon coding_transcript
11967101 11967294 34093 34286 + cds coding_transcript
11967295 11967386 34287 34378 + intron coding_transcript
11967387 11967712 34379 34704 + exon coding_transcript
11967387 11967712 34379 34704 + cds coding_transcript
11967713 11968407 34705 35399 + intron coding_transcript
11968408 11968837 35400 35829 + exon coding_transcript
11968408 11968837 35400 35829 + cds coding_transcript
11968838 11969351 35830 36343 + intron coding_transcript
11969352 11969541 36344 36533 + exon coding_transcript
11969352 11969541 36344 36533 + cds coding_transcript
11969542 11969616 36534 36608 + intron coding_transcript
11969617 11969914 36609 36906 + exon coding_transcript
11969617 11969914 36609 36906 + cds coding_transcript
11969915 11970144 36907 37136 + intron coding_transcript
11970145 11970294 37137 37286 + exon coding_transcript
11970145 11970294 37137 37286 + cds coding_transcript
11970295 11970349 37287 37341 + intron coding_transcript
11970350 11971006 37342 39060 + exon coding_transcript
11970350 11970484 37342 38538 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 37474 37533 + cds 2xTY1 CLC
1548 2264 37540 38256 + cds SGFP CLC
2271 2312 38263 38304 + cds V5 CLC
2313 2336 38305 38328 + cds Precision cut site CLC
2337 2357 38329 38349 + cds TEV CLC
2358 2429 38350 38421 + cds BLRP CLC
3813 3846 38429 38462 + misc_recomb FRT CLC
dmel-5.43-3R
11970485 11971006 38539 39060 + three_prime_utr coding_transcript
11971397 11972577 39451 40631 - gene CG5903 coding_transcript
11972741 11975115 40795 43169 + gene CG5916 coding_transcript
11975291 11977064 43345 45118 - gene Fer2 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Sb[37473]::S000169_fly_pretag_rev
AAACAAATAACAAAAGTAGATCTGCCGTCGTCAGTATCTTCTGATCATCActtgtcgtcgtcatccttgtagtca
fasta Fasta
Sb[37473]::S000169_fly_pretag_fwd
TCTGTACGAGAATCTCCAAGTTTACGCCGTGGATACTGGAGCATGTCAGAgaagtgcataccaatcaggacccgc
fasta Fasta