Clone: 0864533050210965 C11

Tagged gene: CG32249
Identifier: FBgn0052249

Clone FlyFos018953(pRedFlp-Hgr)(CG32249[23440]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
4482809 4484279 9955 11425 - gene CG11350 coding_transcript
4480282 4481487 12747 13952 - gene CG7465 coding_transcript
4473068 4475095 19139 21166 - gene CG11349 coding_transcript
4471758 4472384 21850 22476 - gene CG32248 coding_transcript
4470001 4471012 23222 25295 - gene CG32249 coding_transcript
4470001 4471012 23222 25295 - mrna FBtr0303003 coding_transcript
4470071 4471012 23222 25225 - exon coding_transcript
4470797 4471012 23222 23437 - three_prime_utr coding_transcript
4470071 4470796 23438 25225 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 23514 23547 - misc_recomb FRT CLC
2358 2429 23555 23626 - cds BLRP CLC
2337 2357 23627 23647 - cds TEV CLC
2313 2336 23648 23671 - cds Precision cut site CLC
2271 2312 23672 23713 - cds V5 CLC
1548 2264 23720 24436 - cds SGFP CLC
1482 1541 24443 24502 - cds 2xTY1 CLC
dmel-5.43-3L
4470010 4470070 25226 25286 - intron coding_transcript
4470001 4470009 25287 25295 - exon coding_transcript
4470007 4470009 25287 25289 - cds coding_transcript
4470001 4470006 25290 25295 - five_prime_utr coding_transcript
4465226 4466630 28666 30070 + gene CG32241 coding_transcript
4463048 4463723 31573 32248 + gene CG15024 coding_transcript
4460621 4461339 33957 34675 + gene CG15023 coding_transcript
4459192 4460313 34983 36104 + gene CG15022 coding_transcript
4457320 4458191 37105 37976 + gene CG11345 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG32249[23440]::S000169_fly_pretag_rev
TTGAGTTGGTGGTTGCTCAGAAATGTTGTTTCGGATGATGAGGCATCTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG32249[23440]::S000169_fly_pretag_fwd
ACCTGCCGCCACAAGTCATCGCTCCTCGTAACACCTACCTGCCGCCCCAGgaagtgcataccaatcaggacccgc
fasta Fasta