Clone: 0864533050210965 G10

Tagged gene: Spn88Eb
Identifier: FBgn0038299

Clone FlyFos018948(pRedFlp-Hgr)(Spn88Eb[19071]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
11028791 11032168 15376 18753 - gene Spn5 coding_transcript
11032418 11034484 19003 22131 - gene Spn88Eb coding_transcript
11032418 11034484 19003 22131 - mrna FBtr0083140 coding_transcript
11032418 11032783 19003 20430 - exon coding_transcript
11032418 11032483 19003 19068 - three_prime_utr coding_transcript
11032484 11032783 19069 20430 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19145 19178 - misc_recomb FRT CLC
2358 2429 19186 19257 - cds BLRP CLC
2337 2357 19258 19278 - cds TEV CLC
2313 2336 19279 19302 - cds Precision cut site CLC
2271 2312 19303 19344 - cds V5 CLC
1548 2264 19351 20067 - cds SGFP CLC
1482 1541 20074 20133 - cds 2xTY1 CLC
dmel-5.43-3R
11032784 11032845 20431 20492 - intron coding_transcript
11032846 11033838 20493 21485 - exon coding_transcript
11032846 11033826 20493 21473 - cds coding_transcript
11033827 11033838 21474 21485 - five_prime_utr coding_transcript
11033839 11034405 21486 22052 - intron coding_transcript
11034406 11034484 22053 22131 - exon coding_transcript
11034406 11034484 22053 22131 - five_prime_utr coding_transcript
11034916 11037069 22563 24716 + gene CG4203 coding_transcript
11036999 11039380 24646 27027 - gene CG6654 coding_transcript
11039696 11040567 27343 28214 + gene CG4210 coding_transcript
11040484 11044596 28131 32243 - gene SIDL coding_transcript
11044984 11048093 32631 35740 + gene CG12241 coding_transcript
11047971 11048849 35618 36496 - gene Rpb7 coding_transcript
11049091 11051144 36738 38791 - gene CG31344 coding_transcript
11051289 11053286 38936 40933 + gene Caf1 coding_transcript
11053327 11055161 40974 42808 - gene Art3 coding_transcript
11055378 11056238 43025 43885 + gene mRpS10 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Spn88Eb[19071]::S000169_fly_pretag_fwd
ACACGATTCTCTTTGCGGGCGTCTACAGCGATCCCAGGCAAATGCAGCACgaagtgcataccaatcaggacccgc
fasta Fasta
Spn88Eb[19071]::S000169_fly_pretag_rev
ATATTTTTCTTCAAGTTTTTCAAAAACACACAAGTCTTAAAACACTTCTActtgtcgtcgtcatccttgtagtca
fasta Fasta