Clone: 0864533050210965 E10

Tagged gene: Sk1
Identifier: FBgn0030300

Clone FlyFos018946(pRedFlp-Hgr)(Sk1[30046]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
11324559 11348335 15017 39855 - gene CG11727 coding_transcript
11336574 11340045 27032 31565 + gene Sk1 coding_transcript
11336574 11340045 27032 31565 + mrna FBtr0073497 coding_transcript
11336574 11336741 27032 27199 + exon coding_transcript
11336574 11336741 27032 27199 + five_prime_utr coding_transcript
11336742 11337599 27200 28057 + intron coding_transcript
11337572 11340043 28030 31563 + mrna FBtr0073498 coding_transcript
11337572 11340043 28030 31563 + exon coding_transcript
11337572 11337665 28030 28123 + five_prime_utr coding_transcript
11337600 11340045 28058 31565 + exon coding_transcript
11337600 11337665 28058 28123 + five_prime_utr coding_transcript
11337666 11339591 28124 31111 + cds coding_transcript
11337666 11339591 28124 31111 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 30047 30106 + cds 2xTY1 CLC
1548 2264 30113 30829 + cds SGFP CLC
2271 2312 30836 30877 + cds V5 CLC
2313 2336 30878 30901 + cds Precision cut site CLC
2337 2357 30902 30922 + cds TEV CLC
2358 2429 30923 30994 + cds BLRP CLC
3813 3846 31002 31035 + misc_recomb FRT CLC
dmel-5.43-X
11339592 11340045 31112 31565 + three_prime_utr coding_transcript
11339592 11340043 31112 31563 + three_prime_utr coding_transcript
11348824 11352345 40344 43865 + gene HP5 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Sk1[30046]::S000169_fly_pretag_rev
TCTCGTTATGATTAAGGCTTTGCAAAGCACACGGATTTAATATTTCACTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Sk1[30046]::S000169_fly_pretag_fwd
AGGCGGAGGTTATGCCCGGCCTGATCAATGTGATGACCACCAGTGGGCAGgaagtgcataccaatcaggacccgc
fasta Fasta