Clone: 0864533050210965 B10

Tagged gene: spn-F
Identifier: FBgn0086362

Clone FlyFos018944(pRedFlp-Hgr)(spn-F[20492]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]
Whole tag sequencing full download [show/hide details]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
27088350 27089716 12901 14267 - gene CG18673 coding_transcript
27085969 27087389 15228 16648 - gene CG18672 coding_transcript
27083321 27085771 16846 19296 - gene CG3669 coding_transcript
27080864 27082388 20229 22815 - gene spn-F coding_transcript
27080864 27082388 20229 22815 - mrna FBtr0085760 coding_transcript
27081760 27082388 20229 21919 - exon coding_transcript
27082128 27082388 20229 20489 - three_prime_utr coding_transcript
27081760 27082127 20490 21919 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 20566 20599 - misc_recomb FRT CLC
2358 2429 20607 20678 - cds BLRP CLC
2337 2357 20679 20699 - cds TEV CLC
2313 2336 20700 20723 - cds Precision cut site CLC
2271 2312 20724 20765 - cds V5 CLC
1548 2264 20772 21488 - cds SGFP CLC
1482 1541 21495 21554 - cds 2xTY1 CLC
dmel-5.43-3R
27081692 27081759 21920 21987 - intron coding_transcript
27080864 27081691 21988 22815 - exon coding_transcript
27080965 27081691 21988 22714 - cds coding_transcript
27080864 27080964 22715 22815 - five_prime_utr coding_transcript
27079373 27080622 23057 24306 + gene CG1750 coding_transcript
27078624 27079408 24271 25055 - gene mRpL32 coding_transcript
27061751 27078326 25353 41928 + gene qless coding_transcript
27058342 27061003 42676 45337 + gene CG15564 coding_transcript
27057407 27058058 45621 46272 + gene CG15563 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
spn-F[20492]::S000169_fly_pretag_fwd
ACAGCATTGAGCGCCAGTTTGAGTTCGTTTCCCATGCTGTGGGTGACTTCgaagtgcataccaatcaggacccgc
fasta Fasta
spn-F[20492]::S000169_fly_pretag_rev
CTCCGGTCAGGTCAGAGTTCGGTGGCGAAATAAATGCGCCTACTGGGTCActtgtcgtcgtcatccttgtagtca
fasta Fasta