Clone: 0864533050210965 D09

Tagged gene: Jon99Ciii
Identifier: FBgn0003357

Clone FlyFos018937(pRedFlp-Hgr)(Jon99Ciii[17430]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
25754423 25756108 10081 11766 + gene CG31202 coding_transcript
25752974 25754488 11701 13215 - gene CG15522 coding_transcript
25750948 25751907 14282 15241 + gene Jon99Ci coding_transcript
25749476 25750333 15856 16713 + gene Jon99Cii coding_transcript
25747931 25748823 17366 19320 - gene Jon99Ciii coding_transcript
25747931 25748823 17366 19320 - mrna FBtr0085502 coding_transcript
25747931 25748823 17366 19320 - exon coding_transcript
25748762 25748823 17366 17427 - three_prime_utr coding_transcript
25747964 25748761 17428 19287 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 17504 17537 - misc_recomb FRT CLC
2358 2429 17545 17616 - cds BLRP CLC
2337 2357 17617 17637 - cds TEV CLC
2313 2336 17638 17661 - cds Precision cut site CLC
2271 2312 17662 17703 - cds V5 CLC
1548 2264 17710 18426 - cds SGFP CLC
1482 1541 18433 18492 - cds 2xTY1 CLC
dmel-5.43-3R
25747931 25747963 19288 19320 - five_prime_utr coding_transcript
25740242 25746011 21240 27009 + gene trp coding_transcript
25738707 25740050 27201 28544 + gene capa coding_transcript
25733831 25738614 28637 33420 - gene CG31036 coding_transcript
25731188 25732171 35080 36063 - gene CG15515 coding_transcript
25728986 25729523 37728 38265 + gene CG15517 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Jon99Ciii[17430]::S000169_fly_pretag_fwd
GCGTCACCGGATACCTGGACTGGATCCGCGACAACACCGGCATCTCCTACgaagtgcataccaatcaggacccgc
fasta Fasta
Jon99Ciii[17430]::S000169_fly_pretag_rev
TAATCGTTTATTGAAGAAACCCCTCAGAATTAAGGAACATCAACTGCTTActtgtcgtcgtcatccttgtagtca
fasta Fasta