Clone: 0864533050210965 A08

Tagged gene: CG33127
Identifier: FBgn0053127

Clone FlyFos018925(pRedFlp-Hgr)(CG33127[21631]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
329991 332335 12279 14623 + gene CG31921 coding_transcript
325520 329761 14853 19094 + gene CG31920 coding_transcript
327429 328518 16096 17185 + gene CG33992 coding_transcript
322101 323091 21523 23575 - gene CG33127 coding_transcript
322101 323091 21523 23575 - mrna FBtr0078052 coding_transcript
322101 323091 21523 23575 - exon coding_transcript
322986 323091 21523 21628 - three_prime_utr coding_transcript
322131 322985 21629 23545 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 21705 21738 - misc_recomb FRT CLC
2358 2429 21746 21817 - cds BLRP CLC
2337 2357 21818 21838 - cds TEV CLC
2313 2336 21839 21862 - cds Precision cut site CLC
2271 2312 21863 21904 - cds V5 CLC
1548 2264 21911 22627 - cds SGFP CLC
1482 1541 22634 22693 - cds 2xTY1 CLC
dmel-5.43-2L
322101 322130 23546 23575 - five_prime_utr coding_transcript
320279 321246 24430 25397 + gene CG11911 coding_transcript
318362 319254 26422 27314 + gene CG11912 coding_transcript
297882 303955 41721 47794 - gene Pi3K21B coding_transcript
295109 297413 48263 50567 - gene Hop coding_transcript
293337 294679 50997 52339 + gene U2af38 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG33127[21631]::S000169_fly_pretag_rev
TACTGCTAGAGTATTACGCAAAGATCTCCCCTTCGACACCACGACTCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG33127[21631]::S000169_fly_pretag_fwd
CTTACATTGGATGGATCCACCAAACGATCGGCGCCTACTACCAGCTTAACgaagtgcataccaatcaggacccgc
fasta Fasta