Clone: 8689038524324187 F12

Tagged gene: CG42703
Identifier: FBgn0261614

Clone FlyFos018532(pRedFlp-Hgr)(CG42703[22600]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
18977626 18981785 11303 15462 - gene CG9876 coding_transcript
18987580 18987982 21257 21659 - gene RpL37b coding_transcript
18988755 18989224 22432 23963 - gene CG42703 coding_transcript
18988755 18989224 22432 23963 - mrna FBtr0302953 coding_transcript
18988755 18989146 22432 23885 - exon coding_transcript
18988755 18988920 22432 22597 - three_prime_utr coding_transcript
18988921 18989146 22598 23885 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 22674 22707 - misc_recomb FRT CLC
2358 2429 22715 22786 - cds BLRP CLC
2337 2357 22787 22807 - cds TEV CLC
2313 2336 22808 22831 - cds Precision cut site CLC
2271 2312 22832 22873 - cds V5 CLC
1548 2264 22880 23596 - cds SGFP CLC
1482 1541 23603 23662 - cds 2xTY1 CLC
dmel-5.43-2R
18989147 18989204 23886 23943 - intron coding_transcript
18989205 18989224 23944 23963 - exon coding_transcript
18989205 18989215 23944 23954 - cds coding_transcript
18989216 18989224 23955 23963 - five_prime_utr coding_transcript
18990000 18991256 24739 25995 - gene Gr59c coding_transcript
18991464 18992692 26203 27431 - gene Gr59d coding_transcript
19011517 19013565 46256 48304 - gene Klp59C coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG42703[22600]::S000169_fly_pretag_fwd
AGGCCCGGAATCTTGCCATTTGGCGTGAACCTGATTATCGATACTGCAAGgaagtgcataccaatcaggacccgc
fasta Fasta
CG42703[22600]::S000169_fly_pretag_rev
GGGATCGATCCTTTACGTATTATAATCTGTGGTGACTGCCTTAAAGGTCActtgtcgtcgtcatccttgtagtca
fasta Fasta