Clone: 2743147439044672 A11

Tagged gene: Eig71Ej
Identifier: FBgn0014850

Clone FlyFos018377(pRedFlp-Hgr)(Eig71Ej[28061]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
15654967 15655379 26428 26840 - gene CG43082 coding_transcript
15654348 15654903 26904 27459 - gene Eig71Ek coding_transcript
15653649 15654115 27692 29220 + gene Eig71Ej coding_transcript
15653649 15654115 27692 29220 + mrna FBtr0075582 coding_transcript
15653872 15654115 27692 27935 + exon coding_transcript
15654104 15654115 27692 27703 + five_prime_utr coding_transcript
15653872 15654103 27704 27935 + cds coding_transcript
15653808 15653871 27936 27999 + intron coding_transcript
15653649 15653807 28000 29220 + exon coding_transcript
15653743 15653807 28000 29126 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 28062 28121 + cds 2xTY1 CLC
1548 2264 28128 28844 + cds SGFP CLC
2271 2312 28851 28892 + cds V5 CLC
2313 2336 28893 28916 + cds Precision cut site CLC
2337 2357 28917 28937 + cds TEV CLC
2358 2429 28938 29009 + cds BLRP CLC
3813 3846 29017 29050 + misc_recomb FRT CLC
dmel-5.43-3L
15653649 15653742 29127 29220 + three_prime_utr coding_transcript
15652807 15653314 29555 30062 - gene Eig71Ei coding_transcript
15652036 15652552 30317 30833 + gene Eig71Eh coding_transcript
15650728 15651258 31611 32141 - gene Eig71Eg coding_transcript
15649755 15650501 32368 33114 + gene Eig71Ef coding_transcript
15647572 15649110 33759 35297 + gene Eig71Ee coding_transcript
15645644 15646196 36673 37225 - gene Eig71Ed coding_transcript
15644579 15645354 37515 38290 + gene Eig71Ec coding_transcript
15643991 15644426 38443 38878 - gene CG43084 coding_transcript
15643036 15643641 39228 39833 - gene Eig71Eb coding_transcript
15642220 15642761 40108 40649 + gene Eig71Ea coding_transcript
15641676 15642193 40676 41193 - gene CG43083 coding_transcript

Sets

Oligos

Name/Sequence Export
Eig71Ej[28061]::S000169_fly_pretag_rev
TTTAAAAAGACAAATAGCTTTAAATTTCTCTCAAGAGCTAAGAAAAATCActtgtcgtcgtcatccttgtagtca
 fasta Fasta
Eig71Ej[28061]::S000169_fly_pretag_fwd
GACTGGATTGCGAGAATGTGGCTAGACTAACAGGCATGAGACGGATTCGTgaagtgcataccaatcaggacccgc
 fasta Fasta