Clone: 2743147439044672 F06

Tagged gene: Lcp65Ag2
Identifier: FBgn0020637

Clone FlyFos018338(pRedFlp-Hgr)(Lcp65Ag2[18055]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
6123485 6124086 15284 15885 - gene Lcp65Ag3 coding_transcript
6124413 6125209 16212 17008 - gene Cpr65Au coding_transcript
6126089 6126700 17888 19561 - gene Lcp65Ag2 coding_transcript
6126089 6126700 17888 19561 - mrna FBtr0077059 coding_transcript
6126089 6126452 17888 19313 - exon coding_transcript
6126089 6126253 17888 18052 - three_prime_utr coding_transcript
6126254 6126452 18053 19313 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 18129 18162 - misc_recomb FRT CLC
2358 2429 18170 18241 - cds BLRP CLC
2337 2357 18242 18262 - cds TEV CLC
2313 2336 18263 18286 - cds Precision cut site CLC
2271 2312 18287 18328 - cds V5 CLC
1548 2264 18335 19051 - cds SGFP CLC
1482 1541 19058 19117 - cds 2xTY1 CLC
dmel-5.43-3L
6126453 6126513 19314 19374 - intron coding_transcript
6126514 6126700 19375 19561 - exon coding_transcript
6126514 6126632 19375 19493 - cds coding_transcript
6126633 6126700 19494 19561 - five_prime_utr coding_transcript
6127797 6128370 20658 21231 - gene Lcp65Ag1 coding_transcript
6129404 6129871 22265 22732 - gene Lcp65Af coding_transcript
6130819 6131178 23680 24039 - gene Lcp65Ae coding_transcript
6132323 6133073 25184 25934 - gene Cpr65Av coding_transcript
6135003 6135483 27864 28344 - gene Cpr65Aw coding_transcript
6136507 6137106 29368 29967 + gene Lcp65Ad coding_transcript
6137924 6138493 30785 31354 + gene Lcp65Ac coding_transcript
6139771 6140189 32632 33050 + gene Lcp65Ab2 coding_transcript
6140631 6141084 33492 33945 - gene Cpr65Ax1 coding_transcript
6142648 6143060 35509 35921 + gene Lcp65Ab1 coding_transcript
6143502 6143955 36363 36816 - gene Cpr65Ax2 coding_transcript
6144404 6144741 37265 37602 + gene Lcp65Aa coding_transcript
6145150 6145845 38011 38706 - gene Acp65Aa coding_transcript
6149695 6150473 42556 43334 - gene CG13297 coding_transcript
6151175 6151730 44036 44591 - gene Cpr65Ay coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Lcp65Ag2[18055]::S000169_fly_pretag_rev
GTTGGATAGTATGGCTTTAAAATTGCTAAAGGTCAAAGCAATTTCACTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Lcp65Ag2[18055]::S000169_fly_pretag_fwd
AGAACGGATTCCAGCCCCAGGGTGCTCATCTGCCCGTTGCCCCCGTGGCCgaagtgcataccaatcaggacccgc
fasta Fasta