Clone: 2743147439044672 D03
Tagged gene: CG5466
Identifier: FBgn0038815
| Clone | FlyFos018281(pRedFlp-Hgr)(CG5466[15910]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | bad | |
| Whole tag sequencing | flipped | download [show/hide details] |
GENOME<<>>TAG TAG<<>>GENOME
PREDICTED GGGCAACGTGGGATCAGGAGTGAGCGCGGGAGTAGGAGTGGGTGTCCCTGCAGGACTTAACCTGGGCGCCGCCTTGGGTCTGCCATCGGGCAACATGCACGAAGTGCATACCAATCAGGACCCGCTGGACGAGGTTCACACAAACCAAGATCCACTTGATGAATTCATGGTGTCCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGATGGCGACGTGAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTAGATGCGGGCTGGAGCTCATCGGGTAGCATGCAATAGCTCCGGATTTATCCACTCACATTCTTCCAGCAGTTAGAGAACACTTAGTACTTACACGCAGA
COVERAGE 00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000011111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
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EV5 GGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTGCTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGT
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Genes in pFlyFos vector
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-3R | ||||||||
| 16443632 | 16446990 | 14995 | 19415 | - | gene | CG5466 | coding_transcript | |
| 16443632 | 16446990 | 14995 | 19415 | - | mrna | FBtr0083913 | coding_transcript | |
| 16445884 | 16446990 | 14995 | 17163 | - | exon | coding_transcript | ||
| 16446078 | 16446990 | 14995 | 15907 | - | three_prime_utr | coding_transcript | ||
| 16445884 | 16446077 | 15908 | 17163 | - | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 2437 | 2470 | 15984 | 16017 | - | misc_recomb | FRT | CLC | |
| 2358 | 2429 | 16025 | 16096 | - | cds | BLRP | CLC | |
| 2337 | 2357 | 16097 | 16117 | - | cds | TEV | CLC | |
| 2313 | 2336 | 16118 | 16141 | - | cds | Precision cut site | CLC | |
| 2271 | 2312 | 16142 | 16183 | - | cds | V5 | CLC | |
| 1548 | 2264 | 16190 | 16906 | - | cds | SGFP | CLC | |
| 1482 | 1541 | 16913 | 16972 | - | cds | 2xTY1 | CLC | |
| dmel-5.43-3R | ||||||||
| 16445805 | 16445883 | 17164 | 17242 | - | intron | coding_transcript | ||
| 16445584 | 16445804 | 17243 | 17463 | - | exon | coding_transcript | ||
| 16445584 | 16445804 | 17243 | 17463 | - | cds | coding_transcript | ||
| 16445351 | 16445583 | 17464 | 17696 | - | intron | coding_transcript | ||
| 16444286 | 16445350 | 17697 | 18761 | - | exon | coding_transcript | ||
| 16444286 | 16445350 | 17697 | 18761 | - | cds | coding_transcript | ||
| 16444230 | 16444285 | 18762 | 18817 | - | intron | coding_transcript | ||
| 16443937 | 16444229 | 18818 | 19110 | - | exon | coding_transcript | ||
| 16443937 | 16444229 | 18818 | 19110 | - | cds | coding_transcript | ||
| 16443825 | 16443936 | 19111 | 19222 | - | intron | coding_transcript | ||
| 16443632 | 16443824 | 19223 | 19415 | - | exon | coding_transcript | ||
| 16443813 | 16443824 | 19223 | 19234 | - | cds | coding_transcript | ||
| 16443632 | 16443812 | 19235 | 19415 | - | five_prime_utr | coding_transcript | ||
| 16436973 | 16442619 | 20428 | 26074 | + | gene | CG15923 | coding_transcript | |
| 16418881 | 16438262 | 24785 | 44166 | - | gene | bon | coding_transcript | |
Sets
Oligos
| Name/Sequence | Export |
|---|---|
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CG5466[15910]::S000169_fly_pretag_fwd
CAGGACTTAACCTGGGCGCCGCCTTGGGTCTGCCATCGGGCAACATGCACgaagtgcataccaatcaggacccgc
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Fasta
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CG5466[15910]::S000169_fly_pretag_rev
ATAAATCCGGAGCTATTGCATGCTACCCGATGAGCTCCAGCCCGCATCTActtgtcgtcgtcatccttgtagtca
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Fasta
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