Clone: 8882336809946705 B09

Tagged gene: nimB3
Identifier: FBgn0054003

Clone FlyFos017120(pRedFlp-Hgr)(nimB3[22397]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
13977303 13979573 10314 12584 - gene Cyp28a5 coding_transcript
13974119 13976753 13134 15768 + gene nimC1 coding_transcript
13972096 13972947 16940 17791 + gene He coding_transcript
13970529 13972173 17714 19358 - gene nimB5 coding_transcript
13968280 13969973 19914 21607 + gene nimB4 coding_transcript
13967464 13967966 21921 23485 + gene nimB3 coding_transcript
13967464 13967966 21921 23485 + mrna FBtr0100058 coding_transcript
13967751 13967966 21921 22136 + exon coding_transcript
13967912 13967966 21921 21975 + five_prime_utr coding_transcript
13967751 13967911 21976 22136 + cds coding_transcript
13967695 13967750 22137 22192 + intron coding_transcript
13967464 13967694 22193 23485 + exon coding_transcript
13967487 13967694 22193 23462 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 22398 22457 + cds 2xTY1 CLC
1548 2264 22464 23180 + cds SGFP CLC
2271 2312 23187 23228 + cds V5 CLC
2313 2336 23229 23252 + cds Precision cut site CLC
2337 2357 23253 23273 + cds TEV CLC
2358 2429 23274 23345 + cds BLRP CLC
3813 3846 23353 23386 + misc_recomb FRT CLC
dmel-5.43-2L
13967464 13967486 23463 23485 + three_prime_utr coding_transcript
13965012 13967022 23927 25937 + gene nimB2 coding_transcript
13963521 13965088 25861 27428 - gene nimB1 coding_transcript
13957539 13963381 27568 33410 - gene nimA coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
nimB3[22397]::S000169_fly_pretag_fwd
GTGCTCCGGGTTACTATCGGAGTAACAAAAGGTGTCGTTTTGTCTTGGAAgaagtgcataccaatcaggacccgc
 fasta Fasta
nimB3[22397]::S000169_fly_pretag_rev
AAAGTAAGCAACTCAAAAAATATTGTAATTGATATTATTTATTTTTGTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta