Clone: 10123066965770744 G11

Tagged gene: CG6421
Identifier: FBgn0025827

Clone FlyFos017037(pRedFlp-Hgr)(CG6421[19692]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
12761284 12762453 17590 18759 - gene CG6435 coding_transcript
12760522 12761266 18777 19521 - gene CG6429 coding_transcript
12759755 12760413 19630 21350 - gene CG6421 coding_transcript
12759755 12760413 19630 21350 - mrna FBtr0087043 coding_transcript
12759938 12760413 19630 21167 - exon coding_transcript
12760354 12760413 19630 19689 - three_prime_utr coding_transcript
12759938 12760353 19690 21167 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19766 19799 - misc_recomb FRT CLC
2358 2429 19807 19878 - cds BLRP CLC
2337 2357 19879 19899 - cds TEV CLC
2313 2336 19900 19923 - cds Precision cut site CLC
2271 2312 19924 19965 - cds V5 CLC
1548 2264 19972 20688 - cds SGFP CLC
1482 1541 20695 20754 - cds 2xTY1 CLC
dmel-5.43-2R
12759881 12759937 21168 21224 - intron coding_transcript
12759755 12759880 21225 21350 - exon coding_transcript
12759811 12759880 21225 21294 - cds coding_transcript
12759755 12759810 21295 21350 - five_prime_utr coding_transcript
12756897 12759359 21746 24208 - gene CG6426 coding_transcript
12755698 12756681 24424 25407 - gene Ef1beta coding_transcript
12750470 12755218 25887 30635 + gene Psi coding_transcript
12743726 12747861 33244 37379 - gene Dek coding_transcript
12741482 12742843 38262 39623 + gene Fen1 coding_transcript
12737963 12741606 39499 43142 - gene IntS8 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG6421[19692]::S000169_fly_pretag_fwd
AGAGTGTGTTCGAGGAGTGCATCGAGAGGTACGAGGATGAGGGATTTGAGgaagtgcataccaatcaggacccgc
fasta Fasta
CG6421[19692]::S000169_fly_pretag_rev
AAAAATATTAAGTTTATTAACATATATATATAATGTTTATTTTTTATTTActtgtcgtcgtcatccttgtagtca
fasta Fasta