Clone: 10123066965770744 H09

Tagged gene: PGRP-LF
Identifier: FBgn0035977

Clone FlyFos017016(pRedFlp-Hgr)(PGRP-LF[46374]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
9327432 9331436 29388 33392 + gene PGRP-LA coding_transcript
9331910 9341436 33866 43392 + gene PGRP-LC coding_transcript
9342711 9344589 44667 47607 + gene PGRP-LF coding_transcript
9342711 9344589 44667 47607 + mrna FBtr0076439 coding_transcript
9342711 9342924 44667 44880 + exon coding_transcript
9342711 9342785 44667 44741 + five_prime_utr coding_transcript
9342786 9342924 44742 44880 + cds coding_transcript
9342925 9342995 44881 44951 + intron coding_transcript
9342996 9343147 44952 45103 + exon coding_transcript
9342996 9343147 44952 45103 + cds coding_transcript
9343148 9343543 45104 45499 + intron coding_transcript
9343544 9343928 45500 45884 + exon coding_transcript
9343544 9343928 45500 45884 + cds coding_transcript
9343929 9343987 45885 45943 + intron coding_transcript
9343988 9344589 45944 47607 + exon coding_transcript
9343988 9344421 45944 47439 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 46375 46434 + cds 2xTY1 CLC
1548 2264 46441 47157 + cds SGFP CLC
2271 2312 47164 47205 + cds V5 CLC
2313 2336 47206 47229 + cds Precision cut site CLC
2337 2357 47230 47250 + cds TEV CLC
2358 2429 47251 47322 + cds BLRP CLC
3813 3846 47330 47363 + misc_recomb FRT CLC
dmel-5.43-3L
9344422 9344589 47440 47607 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
PGRP-LF[46374]::S000169_fly_pretag_fwd
TTGGTCATATAAGCAAGAACTATAGTCTAATTGACGATTTAGAAAAGTCAgaagtgcataccaatcaggacccgc
fasta Fasta
PGRP-LF[46374]::S000169_fly_pretag_rev
CACAGGAATGGCACTAGCCAAGTTTCATTCCCCTTGCTTTCAGTTTTCTActtgtcgtcgtcatccttgtagtca
fasta Fasta