Clone: 10123066965770744 F09

Tagged gene: alpha-Est2
Identifier: FBgn0015570

Clone FlyFos017010(pRedFlp-Hgr)(alpha-Est2[33345]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
3350289 3354499 12837 17047 - gene alpha-Est6 coding_transcript
3354586 3355963 17134 18511 - gene Rtnl2 coding_transcript
3356478 3358454 19026 21002 + gene alphaTub84D coding_transcript
3359218 3362247 21766 24795 - gene alpha-Est5 coding_transcript
3362409 3364333 24957 26881 - gene alpha-Est4 coding_transcript
3364694 3367349 27242 29897 - gene alpha-Est3 coding_transcript
3367533 3370952 30081 34562 + gene alpha-Est2 coding_transcript
3367533 3370952 30081 34562 + mrna FBtr0310421 coding_transcript
3367533 3367689 30081 30237 + exon coding_transcript
3367533 3367689 30081 30237 + five_prime_utr coding_transcript
3367690 3368441 30238 30989 + intron coding_transcript
3368442 3368618 30990 31166 + exon coding_transcript
3368442 3368571 30990 31119 + five_prime_utr coding_transcript
3368467 3370952 31015 34562 + mrna FBtr0081540 coding_transcript
3368467 3368618 31015 31166 + exon coding_transcript
3368467 3368571 31015 31119 + five_prime_utr coding_transcript
3368572 3368618 31120 31166 + cds coding_transcript
3368572 3368618 31120 31166 + cds coding_transcript
3368619 3368758 31167 31306 + intron coding_transcript
3368619 3368758 31167 31306 + intron coding_transcript
3368759 3369225 31307 31773 + exon coding_transcript
3368759 3369225 31307 31773 + cds coding_transcript
3368759 3369225 31307 31773 + exon coding_transcript
3368759 3369225 31307 31773 + cds coding_transcript
3369226 3369613 31774 32161 + intron coding_transcript
3369226 3369613 31774 32161 + intron coding_transcript
3369614 3370952 32162 34562 + exon coding_transcript
3369614 3370952 32162 34562 + exon coding_transcript
3369614 3370800 32162 34410 + cds coding_transcript
3369614 3370800 32162 34410 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 33346 33405 + cds 2xTY1 CLC
1548 2264 33412 34128 + cds SGFP CLC
2271 2312 34135 34176 + cds V5 CLC
2313 2336 34177 34200 + cds Precision cut site CLC
2337 2357 34201 34221 + cds TEV CLC
2358 2429 34222 34293 + cds BLRP CLC
3813 3846 34301 34334 + misc_recomb FRT CLC
dmel-5.43-3R
3370801 3370952 34411 34562 + three_prime_utr coding_transcript
3370801 3370952 34411 34562 + three_prime_utr coding_transcript
3371455 3374844 35065 38454 - gene alpha-Est1 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
alpha-Est2[33345]::S000169_fly_pretag_rev
TAGAACATCTGGAGCACTTATATTAACCTTAGAATTAGATTTGAGAACTActtgtcgtcgtcatccttgtagtca
fasta Fasta
alpha-Est2[33345]::S000169_fly_pretag_fwd
CCGATTGCCTAGCCGTTTGGGATACATTTTATCCCAGGGAGTCGCTCTTCgaagtgcataccaatcaggacccgc
fasta Fasta